Mitochondrial mechanism of microvascular endothelial cells apoptosis in hyperhomocysteinemia

Journal of Cellular Biochemistry
Neetu TyagiSuresh C Tyagi

Abstract

An elevated level of homocysteine (Hcy) limits the growth and induces apoptosis. However, the mechanism of Hcy-induced programmed cell death in endothelial cells is largely unknown. We hypothesize that Hcy induces intracellular reactive oxygen species (ROS) production that leads to the loss of transmembrane mitochondrial potential (Deltapsi(m)) accompanied by the release of cytochrome-c from mitochondria. Cytochrome-c release contributes to caspase activation, such as caspase-9, caspase-6, and caspase-3, which results in the degradation of numerous nuclear proteins including poly (ADP-ribose) polymerase (PARP), which subsequently leads to the internucleosomal cleavage of DNA, resulting cell death. In this study, rat heart microvascular endothelial cells (MVEC) were treated with different doses of Hcy at different time intervals. Apoptosis was measured by DNA laddering and transferase-mediated dUTP nick-end labeling (TUNEL) assay. ROS production and MP were determined using fluorescent probes (2,7-dichlorofluorescein (DCFH-DA) and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzamidazolocarbocyanin iodide (JC-1), respectively, by confocal microscopy. Differential gene expression for apoptosis was analyzed by cDNA array. The resul...Continue Reading

References

Mar 1, 1995·European Journal of Clinical Investigation·H J BlomJ M Trijbels
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C TsaiM E Lee
Jun 1, 1993·Free Radical Biology & Medicine·A J Olszewski, K S McCully
Apr 1, 1993·The Journal of Clinical Investigation·J F KeaneyJ Loscalzo
Aug 15, 1997·The Biochemical Journal·G M Cohen
May 14, 1998·Chemistry & Biology·N A Thornberry
May 20, 1998·European Journal of Pediatrics·J D Finkelstein
Aug 26, 1998·Trends in Cell Biology·D Green, G Kroemer
Aug 28, 1998·Science·D R Green, J C Reed
Oct 31, 1998·Oncogene·C Sidoti-de FraisseJ L Vayssière
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Aug 7, 1999·Lancet·G J Hankey, J W Eikelboom
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·K Kuida
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·H ZhangJ C Reed
Apr 27, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C García-RuizJ C Fernández-Checa
May 5, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J G Pastorino, J B Hoek
Oct 26, 2000·Nature·M O Hengartner
Aug 14, 2001·Journal of Cellular Biochemistry·V S MujumdarS C Tyagi
Dec 4, 2003·The Journal of Laboratory and Clinical Medicine·Dinender Kumar, Bodh I Jugdutt
May 1, 2004·Hypertension·Toshimitsu SuharaToshio Ogihara
Jun 10, 2004·World Journal of Gastroenterology : WJG·Cheng Ji, Neil Kaplowitz
May 1, 1990·The Journal of Nutritional Biochemistry·J D Finkelstein
Nov 19, 2004·Nature·Scott W LoweGerard Evan
Feb 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Karni S MoshalSuresh C Tyagi
Aug 9, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Neetu TyagiSuresh C Tyagi

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Citations

Sep 13, 2007·Molecular and Cellular Biochemistry·Neetu TyagiDavid Lominadze
Sep 13, 2011·Cell Biochemistry and Biophysics·Thomas P VacekSuresh C Tyagi
Nov 1, 2011·Cell Biochemistry and Biophysics·Jessica A SipkensHans W M Niessen
Oct 8, 2008·Antioxidants & Redox Signaling·Neetu TyagiSuresh C Tyagi
Dec 4, 2010·Antioxidants & Redox Signaling·Mesia M Steed, Suresh C Tyagi
Sep 29, 2009·Epilepsia·Vincenzo BelcastroFrancesco Pisani
Sep 11, 2008·American Journal of Physiology. Endocrinology and Metabolism·Utpal SenSuresh C Tyagi
Nov 22, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Natia QipshidzeSuresh C Tyagi
Dec 17, 2009·Journal of Biomedical Science·Paolo PudduAntonio Muscari
Feb 13, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Peter AlterBernhard Maisch
Mar 16, 2012·International Journal of Biological Sciences·Natia QipshidzeSuresh C Tyagi
Apr 19, 2007·Apoptosis : an International Journal on Programmed Cell Death·Jessica A SipkensHans W M Niessen
Nov 10, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Neetu TyagiSuresh C Tyagi
Sep 9, 2008·Trends in Molecular Medicine·Eugenio MocchegianiMarco Malavolta
Jun 26, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K FinkH Stopper
Mar 25, 2009·Journal of Cellular Physiology·Neetu TyagiDavid Lominadze
Sep 19, 2006·Thrombosis Research·Derrick L SaulsMaureane Hoffman
May 31, 2014·Oxidative Medicine and Cellular Longevity·Giovanni PaganoAdriana Zatterale
Jun 29, 2015·The International Journal of Biochemistry & Cell Biology·Yunjun XiaoWenhua Ling
Jun 24, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Hao WangBu-Lang Gao
Oct 4, 2011·Medical Hypotheses·Maureane Hoffman
Jun 5, 2007·Biochemical and Biophysical Research Communications·Sandra LevrandLucas Liaudet
Mar 31, 2015·Indian Journal of Pharmacology·Xinming LuoJing Zhao
Apr 8, 2017·Molecular and Cellular Biochemistry·Anastasia FamiltsevaSuresh C Tyagi
Dec 23, 2016·Archives of Biochemistry and Biophysics·Gurumayum Suraj Sharma, Laishram Rajendrakumar Singh
Sep 2, 2008·The International Journal of Lower Extremity Wounds·Elissa M Schwartzfarb, Paolo Romanelli
Aug 24, 2017·Journal of Cellular Physiology·Jonathan C VacekNeetu Tyagi
Jul 25, 2018·Toxins·Maryam AssemLucie Hénaut
Sep 1, 2007·Neuroreport·Keiko ImamuraKenji Nakashima
Aug 1, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Karni S MoshalSuresh C Tyagi
Feb 23, 2010·Journal of Receptor and Signal Transduction Research·Neetu TyagiSuresh C Tyagi
Nov 24, 2017·Physiological Reviews·Guy EelenPeter Carmeliet
Apr 30, 2019·Journal of Cellular Physiology·Yanan KongYu Zhao

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