Mitochondrial membrane potential changes in osteoblasts treated with parathyroid hormone and estradiol

Experimental Cell Research
M B TroyanC V Gay

Abstract

This study assessed mitochondrial membrane potential changes in cultured osteoblasts treated with hormones known to regulate osteoblasts. A fluorescent carbocyanine dye, 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine++ + iodide, also called JC-1, was used as a probe. JC-1 emits photons at 585 nm (orange-red) when the membrane potential in mitochondria is highly negative, but when the potential becomes reduced emission occurs at 527 nm (green). Osteoblasts were rinsed in serum-free medium for 5 min, then loaded with 1 x 10(-6) M JC-1 for 10 min. The distribution and intensity of JC-1 fluorescence were evaluated with a laser-scanning confocal microscope system. Hormone treatments included parathyroid hormone (PTH; 10(-8) M), 17beta-estradiol (10(-8) M), and thyroxine (T4; 10(-8) M). The potassium ionophore valinomycin (10(-6) M) was used as a control since it is known to disrupt the electrochemical gradient of mitochondria without interfering with the pH gradient. Valinomycin caused a profound, rapid increase (22.5% above untreated values) in the green/red ratio, which indicated a lowering of the mitochondrial membrane potential in all samples evaluated. PTH caused a less pronounced, but significant (7-14%), ...Continue Reading

References

Mar 9, 1979·Science·J H Oppenheimer
May 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N Agarwala, C V Gay
Oct 1, 1990·Archives of Biochemistry and Biophysics·R Laethem, J E Zull
Apr 1, 1990·Physiological Reviews·J G McCormackR M Denton
Jan 1, 1988·Annual Review of Cell Biology·G Attardi, G Schatz
Jan 31, 1989·Biochemical and Biophysical Research Communications·T K GrayD J Baylink
Jan 1, 1988·Annual Review of Cell Biology·L B Chen
Jan 1, 1987·Annual Review of Biochemistry·E Carafoli
Jul 1, 1974·Endocrinology·R E Nordquist, M A Palmieri
May 1, 1994·Comparative Biochemistry and Physiology. Comparative Physiology·M R HallC V Gay
Jul 1, 1994·Journal of Cellular Biochemistry·N C PartridgeA T Pearman
Jun 1, 1994·In Vitro Cellular & Developmental Biology. Animal·C V GayV R Gilman
Aug 1, 1993·Biochemical Society Transactions·S Soboll
Nov 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M LieberherrS Balsan
Aug 16, 1993·Biochimica Et Biophysica Acta·S Soboll
Mar 1, 1996·Clinical Orthopaedics and Related Research·G R Mundy

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Citations

Dec 1, 2004·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·F McDonald
Apr 11, 2007·Aquatic Toxicology·Sandra S SoaresManuel Aureliano
Mar 14, 2007·Journal of Inorganic Biochemistry·S S SoaresM Aureliano
Feb 27, 2007·International Immunopharmacology·Genhong YaoPeiyuan Zhu
Sep 28, 2013·The International Journal of Biochemistry & Cell Biology·Bin YuMeifeng Xu
Sep 23, 2011·Toxicology and Industrial Health·Peng LiaoYucheng Sun
Dec 19, 2017·Biotechnology and Bioengineering·Anil B ShriraoBonnie L Firestein
Mar 14, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Vivek Garg, Keli Hu
Jul 20, 2002·Blood·Maher AlbitarElihu H Estey
Sep 26, 2000·American Journal of Physiology. Cell Physiology·S V KomarovaR K Globus
Aug 22, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M XuM Ashraf

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