PMID: 11334787May 4, 2001Paper

Mitochondrial membrane potential in density-separated trout erythrocytes exposed to oxidative stress in vitro

Biochimica Et Biophysica Acta
L TianoG Falcioni

Abstract

Previous literature reports have demonstrated that nucleated trout erythrocytes in condition of oxidative stress are subjected to DNA and membrane damage, and inactivation of glutathione peroxidase. The present study was undertaken to investigate if mitochondrial membrane potential in stressed conditions was also influenced. Density-separated trout erythrocyte fractions, obtained using a discontinuous Percoll gradient, were submitted to stress conditions and the mitochondrial membrane potential was determined by means of cytofluorimetric analysis after incubation of each subfraction with JC-1, a mitochondrial specific fluorescent probe. The results clearly show that the mitochondrial membrane potential decreased significantly in all erythrocyte fractions, also if the oxidative effect on mitochondria is more severe with increased density (age) of the cell. Ebselen was very effective in preventing mitochondrial depolarization in young as well as in old erythrocytes.

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Citations

Oct 31, 2015·Aquatic Toxicology·Knut Erik TollefsenHans-Christian Teien
Jul 25, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Thomas ZulianiMarie-Hélène Ratinaud
Feb 3, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Reiko NagasakaHideki Ushio
Jul 26, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Grazyna Wilczek
May 31, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Agnieszka BabczyńskaIlona Witas
Aug 17, 2002·Biochimica Et Biophysica Acta·C D MoyesB L Tufts
Jan 18, 2005·Ultrasound in Medicine & Biology·Katarzyna MilowskaAndrzej Nowicki
Nov 28, 2018·Journal of Ophthalmic & Vision Research·Mitra FarnoodianNader Sheibani

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