Mitochondrial metabolic states regulate nitric oxide and hydrogen peroxide diffusion to the cytosol

Biochimica Et Biophysica Acta
Alberto BoverisJuanita Bustamante

Abstract

Mitochondria isolated from rat heart, liver, kidney and brain (respiratory control 4.0-6.5) release NO and H2O2 at rates that depend on the mitochondrial metabolic state: releases are higher in state 4, about 1.7-2.0 times for NO and 4-16 times for H2O2, than in state 3. NO release in rat liver mitochondria showed an exponential dependence on membrane potential in the range 55 to 180 mV, as determined by Rh-123 fluorescence. A similar behavior was reported for mitochondrial H2O2 production by [S.S. Korshunov, V.P. Skulachev, A.A. Starkov, High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria. FEBS Lett. 416 (1997) 15_18.]. Transition from state 4 to state 3 of brain cortex mitochondria was associated to a decrease in NO release (50%) and in membrane potential (24-53%), this latter determined by flow cytometry and DiOC6 and JC-1 fluorescence. The fraction of cytosolic NO provided by diffusion from mitochondria was 61% in heart, 47% in liver, 30% in kidney, and 18% in brain. The data supports the speculation that NO and H2O2 report a high mitochondrial energy charge to the cytosol. Regulation of mtNOS activity by membrane potential makes mtNOS a regulable enzyme that in turn regulat...Continue Reading

References

Dec 1, 1970·Archives of Biochemistry and Biophysics·A Boveris, A O Stoppani
Jul 1, 1972·The Biochemical Journal·A BoverisB Chance
Apr 1, 1996·Archives of Biochemistry and Biophysics·A DenicolaE Lissi
Jan 6, 1999·Biophysical Journal·R C Scaduto, L W Grotyohann
Dec 23, 1999·The Journal of Biological Chemistry·J J PoderosoA Boveris
May 16, 2002·Nitric Oxide : Biology and Chemistry·Juanita BustamanteAlberto Boveris
Aug 3, 2002·The Journal of Biological Chemistry·Sarah Liv ElferingCecilia Giulivi
Feb 8, 2003·Science·Enzo NisoliMichele O Carruba
Jul 26, 2003·Antioxidants & Redox Signaling·Alberto BoverisAna Navarro
Aug 13, 2003·Journal of Neurochemistry·Anatoly A Starkov, Gary Fiskum
Jul 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·María C CarrerasJuan J Poderoso
Aug 12, 2004·Free Radical Biology & Medicine·Victor Darley-Usmar
Sep 17, 2004·Archives of Biochemistry and Biophysics·Silvia Lores-ArnaizAlberto Boveris
Sep 30, 2004·Free Radical Biology & Medicine·Silvia Alvarez, Alberto Boveris
Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fernando AntunesEnrique Cadenas
Jan 5, 2005·Free Radical Biology & Medicine·Sruti ShivaVictor M Darley-Usmar
Feb 8, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Gustavo F GonzalesAlberto Boveris
Jul 15, 2005·American Journal of Physiology. Endocrinology and Metabolism·Ana NavarroAlberto Boveris
Nov 18, 2005·Methods in Enzymology·Laura B ValdezAlberto Boveris

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Citations

Nov 17, 2009·Journal of Bioenergetics and Biomembranes·Ana Navarro, Alberto Boveris
Oct 7, 2009·Antioxidants & Redox Signaling·Werner J H KoopmanPeter H G M Willems
Nov 4, 2008·Carcinogenesis·Scott MaynardVilhelm A Bohr
Apr 8, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Na ChenYan Xiong
Sep 24, 2010·Journal of Nucleic Acids·Claus DeslerLene Juel Rasmussen
Jul 17, 2012·Antioxidants & Redox Signaling·Fei YinEnrique Cadenas
Sep 26, 2014·Current Atherosclerosis Reports·Lona J Kroese, Peter G Scheffer
Sep 23, 2014·Journal of Bioenergetics and Biomembranes·J BustamanteS Lores-Arnaiz
Oct 26, 2013·Basic Research in Cardiology·Francesca TullioPasquale Pagliaro
Jul 5, 2011·Free Radical Biology & Medicine·Laura B ValdezRicardo J Gelpi
Apr 20, 2010·DNA Repair·Barbara van LoonUlrich Hübscher
Oct 13, 2015·Free Radical Biology & Medicine·Darío E IglesiasAlberto Boveris
Apr 19, 2008·IUBMB Life·Alberto Boveris, Ana Navarro
Feb 3, 2007·Muscle & Nerve·Jennifer S Moylan, Michael B Reid
Aug 19, 2008·Archives of Biochemistry and Biophysics·Philip Y Lam, Enrique Cadenas
Apr 27, 2010·Biochimica Et Biophysica Acta·Andrea DlaskováRichard K Porter
Jun 7, 2015·Archives of Toxicology·Dania C Liemburg-ApersSander Grefte
Oct 6, 2012·The International Journal of Biochemistry & Cell Biology·Kieran J Clarke, Richard K Porter
Nov 27, 2014·Anesthesia and Analgesia·Carlos VenâncioTeresa Summavielle
Jul 6, 2016·Clínica e investigación en arteriosclerosis : publicación oficial de la Sociedad Española de Arteriosclerosis·Alejandra B Camargo, Walter Manucha
Aug 16, 2016·Archives of Biochemistry and Biophysics·Silvina S BombicinoLaura B Valdez
Sep 30, 2016·The International Journal of Biochemistry & Cell Biology·Silvina S BombicinoLaura B Valdez
Nov 12, 2014·European Heart Journal·Alfonso EirinLilach O Lerman
Nov 19, 2014·Hypertension·Alfonso EirinLilach O Lerman
Oct 2, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Tamara Zaobornyj, Pedram Ghafourifar
Dec 24, 2015·Current Hypertension Reports·Marisa Nile MolinaWalter Manucha
Nov 25, 2018·Genes·Belén Mendoza-ChamizoGodefroid Charbon
Jun 14, 2017·Oxidative Medicine and Cellular Longevity·Reza NorouziradAsghar Ghasemi
Jun 25, 2019·The American Journal of Pathology·Swati ShahWilliam Reid
Aug 17, 2021·Frontiers in Physiology·Egor Plotnikov, Giuliano Ciarimboli

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