Mitochondrial-nuclear crosstalk, haplotype and copy number variation distinct in muscle fiber type, mitochondrial respiratory and metabolic enzyme activities

Scientific Reports
Xuan LiuSiriluck Ponsuksili

Abstract

Genes expressed in mitochondria work in concert with those expressed in the nucleus to mediate oxidative phosphorylation (OXPHOS), a process that is relevant for muscle metabolism and meat quality. Mitochondrial genome activity can be efficiently studied and compared in Duroc and Pietrain pigs, which harbor different mitochondrial haplotypes and distinct muscle fiber types, mitochondrial respiratory activities, and fat content. Pietrain pigs homozygous-positive for malignant hyperthermia susceptibility (PiPP) carried only haplotype 8 and showed the lowest absolute mtDNA copy number accompanied by a decrease transcript abundance of mitochondrial-encoded subunits ND1, ND6, and ATP6 and nuclear-encoded subunits NDUFA11 and NDUFB8. In contrast, we found that haplotype 4 of Duroc pigs had significantly higher mitochondrial DNA (mtDNA) copy numbers and an increase transcript abundance of mitochondrial-encoded subunits ND1, ND6, and ATP6. These results suggest that the variation in mitochondrial and nuclear genetic background among these animals has an effect on mitochondrial content and OXPHOS system subunit expression. We observed the co-expression pattern of mitochondrial and nuclear encoded OXPHOS subunits suggesting that the mito...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 11, 2000·Nucleic Acids Research·J HuA L Archibald
Aug 23, 2001·Trends in Molecular Medicine·G L Semenza
Jan 15, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·N GueguenP Herpin
Dec 26, 2006·Molecular Cell·Nicholas D BonawitzGerald S Shadel
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lisa S ShockShirley M Taylor
May 12, 2011·Integrative and Comparative Biology·Yun ShiHolly Van Remmen
Oct 28, 2011·American Journal of Physiology. Cell Physiology·Martin PicardYan Burelle
Mar 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ornella ZolloNeal Sondheimer
Jun 24, 2014·Molecular Systems Biology·Atsushi FukuohHoward T Jacobs
Apr 14, 2015·Genetics and Molecular Research : GMR·Y M XieX W Li
Jul 26, 2015·Meat Science·Tracy L SchefflerDavid E Gerrard
Dec 19, 2015·International Journal of Biological Sciences·Xuan LiuSiriluck Ponsuksili
Jun 28, 2016·Domestic Animal Endocrinology·Tesha Tsai, Justin C St John
Jul 28, 2016·Proceedings of the National Academy of Sciences of the United States of America·Richard G HunterBruce S McEwen
Nov 2, 2016·Therapeutics and Clinical Risk Management·Masoumeh FalahMohammad Farhadi
Feb 20, 2017·Mitochondrion·Tiina S SalminenLaurie S Kaguni

❮ Previous
Next ❯

Citations

Aug 20, 2019·Reproductive Biology and Endocrinology : RB&E·Jingshun ZhangLianwen Zheng
Aug 23, 2019·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Basavaraj SajjanarSiriluck Ponsuksili
Feb 11, 2021·International Journal of Molecular Sciences·Antonio ManciniAndrea Silvestrini
Jul 1, 2021·American Journal of Physiology. Cell Physiology·Paria Ali PourArash Kheradvar

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

R package IdeoViz
DNASP
BLASTN
SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.