Mitochondrial p53 levels parallel total p53 levels independent of stress response in human colorectal carcinoma and glioblastoma cells

Oncogene
Mojgan Mahyar-RoemerKlaus Roemer

Abstract

p53 can eliminate damaged cells through the induction of mitochondria-mediated apoptosis. Recent observations have provided strong evidence that a fraction of total p53 translocates to mitochondria specifically in response to a death stimulus. Unexpectedly, mutant p53, which is expressed at much higher levels than wild type in unstressed cells, is apparently always present at the mitochondria, independent of apoptotic signal. This prompted us to ask whether cell lines with intact p53-dependent apoptosis and cell cycle arrest pathways exist in which the mitochondrial localization of wild-type p53, like that of mutant, is independent of a death stimulus and instead, correlates with the total p53 levels. Here, we document that human HCT116 colorectal carcinoma cells treated with adriamycin or 5-fluorouracil (5FU) can accumulate total p53 to equally high levels, and mitochondrial p53 to proportionate levels, although only 5FU treatment provoked p53-dependent apoptosis. Along the same line, HCT116 derivatives with increased basal p53 levels, and glioblastoma cells with a doxycycline-inducible p53, also revealed proportionate mitochondrial p53 levels, and even unstressed HCT116 cells had some p53 located at the mitochondria. Finally,...Continue Reading

References

Feb 24, 1995·Cell·E M MiyashitaD A Thorley-Lawson
Jun 1, 1995·Molecular and Cellular Biology·L B Owen-SchaubE Kruzel
Nov 5, 1997·Nature Genetics·G S WuW S el-Deiry
Apr 6, 1999·Japanese Journal of Cancer Research : Gann·C Gao, N Tsuchida
Aug 3, 1999·The Journal of Clinical Investigation·F BunzB Vogelstein
May 24, 2000·The Journal of Biological Chemistry·N D MarchenkoU M Moll
Sep 16, 2000·The Journal of Biological Chemistry·H F DingD E Fisher
Jun 5, 2001·Nature Cell Biology·M C MoroniK Helin
Jul 21, 2001·Molecular Cell·K Nakano, K H Vousden
Sep 26, 2001·The Journal of Biological Chemistry·L WangS Liu
Oct 10, 2001·The Journal of Cell Biology·A FortinR S Slack
Jan 10, 2002·Cell·C Prives, J L Manley
Feb 8, 2002·Cell·Ricky W JohnstoneScott W Lowe
Aug 3, 2002·Nature Reviews. Cancer·Karen H Vousden, Xin Lu
Oct 18, 2002·BMC Cancer·Mojgan Mahyar-RoemerKlaus Roemer
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jian YuLin Zhang
Mar 26, 2003·The Journal of Biological Chemistry·Prasad V JallepalliFred Bunz
Apr 2, 2003·Molecular Cell·Motohiro MiharaUte M Moll
May 6, 2003·Biochemical and Biophysical Research Communications·Luca Scorrano, Stanley J Korsmeyer
May 16, 2003·The Journal of Biological Chemistry·Kristina LöhrMatthias Dobbelstein
Aug 30, 2003·Oncogene·George IliakisHuichen Wang

❮ Previous
Next ❯

Citations

Nov 26, 2010·Apoptosis : an International Journal on Programmed Cell Death·Fei WangYunfeng Zhao
Nov 18, 2008·Cell Death and Differentiation·M BakhanashviliG Rahav
Mar 7, 2009·The Journal of Biological Chemistry·Hirohito YamaguchiHong-Gang Wang
Mar 17, 2009·AIDS·Mary BakhanashviliGalia Rahav
Jul 1, 2009·Expert Opinion on Drug Discovery·Pedro Mestres, Andrea Morguet
Apr 22, 2014·FEBS Letters·Anna ComelGiannino Del Sal
Dec 18, 2013·Seminars in Pediatric Neurology·Christos D KatsetosPavel Dráber
Nov 28, 2007·Journal of Neuroscience Research·Junghee LeeHoon Ryu
May 4, 2005·Biochemical and Biophysical Research Communications·Susan Erster, Ute M Moll
Apr 19, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tian-Jye HsiehChing-Hsein Chen
Jan 1, 2014·Oxidative Medicine and Cellular Longevity·Hyeonji KimSomi Kim Cho
Dec 7, 2010·Biochimica Et Biophysica Acta·Liora LindenboimReuven Stein
Nov 3, 2009·Trends in Cell Biology·Daniel Speidel
Nov 2, 2013·Mitochondrion·Nadi T Wickramasekera, Gokul M Das
Mar 19, 2013·Mitochondrion·Christopher A KoczorWilliam Lewis
Feb 22, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ramaneeya NithipongvanitchTerry D Oberley
Jun 13, 2008·Molecular Cancer·Kristina HeyneKlaus Roemer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis