PMID: 11905998Mar 22, 2002Paper

Mitochondrial permeability transition and calcium dynamics in striatal neurons upon intense NMDA receptor activation

Journal of Neurochemistry
Conrad C AlanoShey-Shing Sheu

Abstract

Deregulation of the intracellular Ca2+ homeostasis by NMDA receptor activation leads to neuronal cell death. Induction of the mitochondrial permeability transition pore (MPT) by Ca2+ is a critical event in mediating cell death. In this study, we used fluorescent Ca2+ indicators to investigate the effect of high concentrations of NMDA on cytosolic and mitochondrial Ca2+ concentrations ([Ca2+]c and [Ca2+]m, respectively) in cultured striatal neurons. Exposure to NMDA resulted in an immediate, sustained increase in [Ca2+]c followed by a secondary increase in [Ca2+]c. This second increase of [Ca2+]c was prevented by pretreatment with N-methyl-valine-4-cyclosporin (NMV-Cys). Exposure of neurons to NMDA also resulted in an increase in [Ca2+]m that was followed by a precipitous decrease in the rhod-2 signal. This decrease followed the time frame of the secondary increase in [Ca2+]c. Preincubation of the neurons with NMV-Cys prevented the decrease in rhod-2 fluorescence. These dynamic changes in the rhod-2 signal and [Ca2+]m in response to NMDA were confirmed by using confocal microscopy. The presented results indicate that MPT can be detected in living neurons using fluorescent Ca2+ indicators, which would allow the study of the physi...Continue Reading

Citations

Dec 4, 2003·Neurochemistry International·S S KannurpattiN B Joshi
Jul 21, 2004·The Journal of Experimental Medicine·Irina G StavrovskayaBruce S Kristal
Aug 12, 2003·Journal of Neurotrauma·Jose L Perez VelazquezMarina V Frantseva
Jan 8, 2009·Journal of Neurotrauma·Cassie S Mitchell, Robert H Lee
May 28, 2014·Neuroscience Letters·Regina JakobShey-Shing Sheu
Dec 22, 2015·Mitochondrion·P Mason McClatcheyJane E B Reusch
Jan 20, 2006·The FEBS Journal·Christos Chinopoulos, Vera Adam-Vizi
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Feb 1, 2005·Cell·Daniele BanoPierluigi Nicotera
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Nov 19, 2021·Frontiers in Cellular Neuroscience·Luisa Fernanda Toro-FernándezRafael Posada-Duque

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