Mitochondrial protein sulfenation during aging in the rat brain

Biophysical Reports
Xiaorong YangLiang-Jun Yan

Abstract

There is accumulating evidence that cysteine sulfenation (cys-SOH) in proteins plays an important role in cellular response to oxidative stress. The purpose of the present study was to identify mitochondrial proteins that undergo changes in cys-SOH during aging. Studies were conducted in rats when they were 5 or 30 months of age. Following blocking of free protein thiols with N-ethylmaleimide, protein sulfenic acids were reduced by arsenite to free thiol groups that were subsequently labeled with biotin-maleimide. Samples were then comparatively analyzed by two-dimensional Western blots, and proteins showing changes in sulfenation were selectively identified by mass spectrometry peptide sequencing. As a result, five proteins were identified. Proteins showing an age-related decrease in sulfenation include pyruvate carboxylase and pyruvate dehydrogenase; while those showing an age-related increase in sulfenation include aconitase, mitofilin, and tubulin (α-1). Results of the present study provide a general picture of mitochondrial protein sulfenation in brain oxidative stress and implicate the involvement of protein sulfenation in overall decline of mitochondrial function during brain aging.

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Citations

Sep 28, 2018·Journal of Cellular Physiology·Yansheng FengJean C Bopassa
Feb 12, 2019·Frontiers in Neuroscience·Victor S Van LaarSarah B Berman

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Methods Mentioned

BETA
gel filtration
affinity purification
electrophoresis
affinity capture

Software Mentioned

ProtTech

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