Mitochondrial proteomic characterization of human normal articular chondrocytes

Osteoarthritis and Cartilage
Cristina Ruiz-RomeroF J Blanco

Abstract

Mitochondrial dysfunctions have been associated with apoptosis, aging and osteoarthritis (OA). Chondrocyte mitochondrial proteins are attractive targets for the study of the metabolism of cartilage degradation. The copurification of "contaminating" proteins has been the major problem in all phases of mitochondrial proteome research. Therefore, we set up a procedure for the proteomic analysis of human chondrocyte mitochondrial proteins. Four types of protein extracts were obtained from primary cultured chondrocytes isolated from healthy donors: (1) initial total chondrocyte extract (CE), (2) cytosol-enriched supernatant fraction (CY), (3) crude mitochondria fraction (CM), and (4) pure mitochondria fraction (PM). Mitochondria were purified by density gradient ultracentrifugation. Mitochondrial proteins were separated by means of two-dimensional gel electrophoresis (2-DE) and silver stained. Protein spots were then identified by mass spectrometry using MALDI-TOF/TOF technology. The best 2-DE reference map of mitochondrial proteome was constructed employing PM fraction. Thirty-nine percent of the identified proteins were functionally distributed in the mitochondria, 14% in the endoplasmic reticulum and 36% in the cytoplasm. Examini...Continue Reading

References

Apr 1, 1992·Electrophoresis·T RabilloudC Ettori
Jun 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H MatsumotoI M Shapiro
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J HenzelC Watanabe
Aug 28, 1998·Science·D R Green, J C Reed
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Nov 26, 2002·The Journal of Biological Chemistry·Wei WangThorsten Kirsch
Mar 13, 2003·Arthritis and Rheumatism·Emilia ManeiroFrancisco J Blanco
Sep 18, 2003·Osteoarthritis and Cartilage·Y E HenrotinJ-P L Pujol
Oct 27, 2004·Molecular & Cellular Proteomics : MCP·Kei FukadaHaining Zhu
Dec 3, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Thorsten Kirsch
Dec 16, 2004·Molecular & Cellular Proteomics : MCP·Karim RezaulDavid Han
Jan 14, 2005·Molecular Biology of the Cell·George B JohnJiping Zha
Aug 27, 2005·Mitochondrion·Francisco J BlancoEmilia Maneiro

❮ Previous
Next ❯

Citations

Sep 17, 2009·Molecular BioSystems·Cristina Ruiz-Romero, Francisco J Blanco
Feb 12, 2009·Molecular & Cellular Proteomics : MCP·Anthony C Smith, Alan J Robinson
Apr 5, 2007·Arthritis Research & Therapy·Reuben GobezieDavid M Lee
Jul 31, 2010·International Journal of Clinical Rheumatology·Shawn P Grogan, Darryl D D'Lima
Mar 25, 2014·Experimental and Molecular Pathology·Clémence DesjardinLaurent Schibler
Mar 13, 2014·Rheumatology·Marta N Valcárcel-AresMaría J López-Armada
Jul 17, 2014·International Journal of Molecular Sciences·Silvia Rodríguez-LombarderoM Esperanza Cerdán
Jul 15, 2015·Expert Review of Proteomics·Cristina Ruiz-RomeroFrancisco J Blanco
Nov 1, 2006·Expert Opinion on Drug Discovery·Stijn LambrechtDirk Elewaut
Apr 11, 2012·Bone·Martin Lotz, Richard F Loeser
May 30, 2009·Drug Discovery Today·Frédéric De Ceuninck, Francis Berenbaum
Mar 21, 2007·Biochimica Et Biophysica Acta·Silke HenrichRichard I Christopherson
Feb 18, 2015·Journal of Proteomics·Cristina Ruiz-RomeroFrancisco J Blanco
May 15, 2007·Journal of Cellular Biochemistry·Sara PadidarJanice E Drew
Aug 10, 2011·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Alexander Y HuiRobert L Sah
Sep 5, 2009·Journal of Cellular Biochemistry·Adriano SpreaficoAnnalisa Santucci
Apr 30, 2008·Methods : a Companion to Methods in Enzymology·Richard WilsonJohn F Bateman
May 25, 2016·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P K Sacitharan, T L Vincent
Mar 1, 2008·Proteomics. Clinical Applications·Stijn LambrechtDieter Deforce
Feb 1, 2008·Proteomics. Clinical Applications·Richard Wilson, John F Bateman
Feb 1, 2009·Proteomics. Clinical Applications·Emilio CamafeitaBenjamín Fernández-Gutiérrez
Feb 19, 2013·Expert Review of Proteomics·Mohamad WardaJin Han
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ioannis P TrougakosEfstathios S Gonos
Dec 17, 2020·Frontiers in Medicine·Xingjia MaoChuan Xiang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis