Mitochondrial pyruvate carrier 1 expression controls cancer epithelial-mesenchymal transition and radioresistance

Cancer Science
Yuji TakaokaHideshi Ishii

Abstract

Mitochondrial pyruvate carrier (MPC) is known to cause different expressions in normal and cancer cells. We observed a change in phenotype with the suppression of MPC expression. We knocked down MPC1 and/or MPC2 using siRNA or shRNA. We observed its cell morphology and accompanying molecular marker. Furthermore, the radioresistance of the MPC knockdown cell line was examined using a colony formation assay. MPC1-suppressed cells changed their morphology to a spindle shape. Epithelial-mesenchymal transition (EMT) was suspected, and examination of the EMT marker by PCR showed a decrease in E-cadherin and an increase in fibronectin. Focusing on glutamine metabolism as the mechanism of this phenomenon, we knocked down the glutamine-metabolizing enzyme glutaminase (GLS). EMT was also observed in GLS-suppressed cells. Furthermore, when MPC1-suppressed cells were cultured in a glutamine-deficient medium, changes in EMT markers were suppressed. In addition, MPC1-suppressed cells also increased with a significant difference in radioresistance. Decreased MPC1 expression favorably affects EMT and radioresistance of cancer.

References

Feb 24, 1956·Science·O WARBURG
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
Feb 13, 2015·The EMBO Journal·Tom BenderJean-Claude Martinou
Dec 8, 2016·Scientific Reports·Masaaki MiyoHideshi Ishii
Apr 17, 2017·Oncotarget·Justyna KanskaW Ruprecht Wiedemeyer

❮ Previous
Next ❯

Citations

Jul 28, 2020·Biomolecules·Joséphine ZangariJean-Claude Martinou
Aug 14, 2020·Biomolecules·Jane L Buchanan, Eric B Taylor
Sep 22, 2019·Brain Sciences·Bor Luen Tang
May 8, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Liangchen Qu Youyou Zhu
Oct 27, 2021·Cancer Biology & Therapy·Vinod S BishtKiran Ambatipudi

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE89393
GSE16515

Methods Mentioned

BETA
PCR

Software Mentioned

Wave

Related Concepts

Related Feeds

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.