PMID: 18198400Jan 17, 2008Paper

Mitochondrial recycling of ascorbic acid as a mechanism for regenerating cellular ascorbate

BioFactors
James M MayCharles E Cobb

Abstract

Mitochondria are the major source of potentially damaging reactive oxygen species in most cells. Since ascorbic acid, or vitamin C, can protect against cellular oxidant stress, we studied the ability of mitochondria prepared from guinea pig skeletal muscle to recycle the vitamin from its oxidized forms. Although ascorbate concentrations in freshly prepared mitochondria were only about 0.2 mM, when provided with 6 mM succinate and 1 mM dehydroascorbate (the two-electron-oxidized form of the vitamin), mitochondria were able to generate and maintain concentrations as high as 4 mM, while releasing most of the ascorbate into the incubation medium. Mitochondrial reduction of dehydroascorbate was strongly inhibited by 1,3-bis(chloroethyl)-1-nitrosourea and by phenylarsine oxide. Despite existing evidence that mitochondrial ascorbate protects the organelle from oxidant damage, ascorbate failed to preserve mitochondrial alpha-tocopherol during prolonged incubation in oxygenated buffer. Nonetheless, the capacity for mitochondria to recycle ascorbate from its oxidized forms, measured as ascorbate-dependent ferricyanide reduction, was several-fold greater than total steady-state ascorbate concentrations. This, and the finding that more tha...Continue Reading

References

Sep 30, 1975·Annals of the New York Academy of Sciences·B H BielskiP C Chan
Jan 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K OgawaH Mayahara
Dec 1, 1995·The American Journal of Clinical Nutrition·E NikiN Gotoh
Sep 13, 1995·Biochimica Et Biophysica Acta·J M MayR R Whitesell
May 2, 1995·Biochemistry·A J Ham, D C Liebler
Feb 1, 1996·Journal of Bioenergetics and Biomembranes·D P Xu, W W Wells
Sep 5, 1997·The Journal of Biological Chemistry·J M MayR F Burk
Jul 17, 1998·Free Radical Biology & Medicine·S MendirattaJ M May
Aug 29, 1998·The Journal of Biological Chemistry·J M MayR F Burk
Nov 14, 1998·Biochimica Et Biophysica Acta·I KoshiishiT Imanari
May 25, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J M May
Oct 6, 2000·Free Radical Biology & Medicine·G A DiLabio, J S Wright
May 23, 2001·Archives of Biochemistry and Biophysics·X LiJ M May
Dec 18, 2001·Rapid Communications in Mass Spectrometry : RCM·P PastoreD Marton
Feb 21, 2002·Biochimica Et Biophysica Acta·Oreste Arrigoni, Mario C De Tullio
Feb 20, 2004·American Journal of Physiology. Cell Physiology·James M MayCharles E Cobb
Feb 16, 2005·Biochemical Pharmacology·Hans NohlKatrin Staniek
Aug 27, 2005·Mitochondrion·Xia Li, James M May
Oct 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sagan KCDavid W Golde
Feb 24, 2006·Radiation Research·John E BiaglowJohn J Mieyal

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Citations

May 6, 2011·The Journal of Nutritional Biochemistry·Christopher MasterjohnRichard S Bruno
May 6, 2009·Toxicology Letters·Luisa MartinoVincenzo De Tata
Jun 11, 2009·British Journal of Pharmacology·J MandlG Bánhegyi
Jan 5, 2013·IUBMB Life·Catia AzzoliniOrazio Cantoni
Jul 19, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Mara FioraniOrazio Cantoni
Jul 23, 2014·Free Radical Biology & Medicine·Darius J R Lane, Des R Richardson
Jul 13, 2017·Antioxidants & Redox Signaling·Orazio CantoniMara Fiorani
Jul 13, 2018·Frontiers in Molecular Biosciences·Vito ScaleraAnnamaria Tonazzi
Dec 30, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Nicholas P Illsley, Marc U Baumann

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