Mitochondrial Remodeling in Chicken Induced Pluripotent Stem-Like Cells

Stem Cells and Development
Hyun Woo ChoiJeong Tae Do

Abstract

Chicken pluripotent stem cells (PSCs), such as embryonic stem cells and blastoderm cells, have been used to study development and differentiation in chicken. However, chicken PSCs are not widely used because they are hard to maintain in long-term culture. Recent reports suggest that chicken somatic cells can be reprogrammed to pluripotent state by defined factors to form induced pluripotent stem cells (iPSCs). These chicken iPSCs showed pluripotent differentiation potential and could be maintained in long-term culture. However, intracytoplasmic remodeling during reprogramming of chicken cells remains largely unknown. In this study, we generated chicken iPS-like cells (ciPSLCs) from chicken embryonic fibroblasts using a retroviral expression system encoding human reprogramming factors. These ciPSLCs could be maintained for more than 10 passages and expressed the endogenous chicken pluripotency markers, cNonog and cSox2. Moreover, the ciPSLCs showed higher nucleus to cytoplasm ratio and contained globular mitochondria with immature cristae. This morphology was similar to that of mammalian PSCs, but different from that of avian somatic cells, which showed lower nucleus to cytoplasm ratio and mature mitochondria. These results sugg...Continue Reading

References

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Citations

Sep 2, 2020·Stem Cells and Development·Mi Jin RyuJeong Tae Do
Sep 17, 2019·World Journal of Stem Cells·Laís Vicari de Figueiredo PessôaKristine Karla Freude
May 22, 2021·Food Science of Animal Resources·Tae Kyung HongSung Gu Han

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Methods Mentioned

BETA
electron microscopy

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