Mitochondrial Respiration in Intact Peripheral Blood Mononuclear Cells and Sirtuin 3 Activity in Patients with Movement Disorders

Oxidative Medicine and Cellular Longevity
S MichalakEwa Wysocka

Abstract

Mitochondrial dysfunction is considered a unifying pathophysiological explanation for movement disorders. Sirtuin 3 (SIRT3) exhibits deacetylase activity and antioxidant properties. The aim of the study was to analyze the mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) and the SIRT3 activity in patients with movement disorders. Mitochondrial respiration was analyzed in intact PBMCs using the ROUTINE, LEAK, electron transfer system (ETS), and residual oxygen consumption (ROX) protocol by means of high-resolution respirometry. The SIRT3 expression and PBMC activity were measured using fluorometry. Ultrasound measurements of the echogenicity of the substantia nigra and the diameter of the 3rd ventricle were also performed. Patients with movement disorders exhibited a lower ROUTINE respiration than controls (P = 0.0237). Reduced oxygen fluxes in the LEAK (P = 0.033) and ROX (P = 0.0486) states were observed in patients with movement disorders compared with controls. Decreased ROUTINE respiration (P = 0.007) and oxygen flux in the LEAK state (P = 0.0203) were observed in patients with PD with substantia nigra hyperechogenicity compared with controls. Decreased SIRT 3 deacetylase activity was found in patients...Continue Reading

References

Jan 1, 1992·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·H YoshinoY Mizuno
Apr 1, 1992·Brain : a Journal of Neurology·V M MannC D Marsden
Jan 1, 1992·Movement Disorders : Official Journal of the Movement Disorder Society·D BraviT N Chase
Mar 1, 1990·Journal of Neurochemistry·A H SchapiraC D Marsden
Dec 1, 1989·Annals of Neurology·W D ParkerJ K Parks
Jan 1, 1997·Movement Disorders : Official Journal of the Movement Disorder Society·C I BlakeS J Boyson
Sep 26, 2000·Journal of Neuroscience Research·K KoshimuraY Kato
Sep 29, 2000·Movement Disorders : Official Journal of the Movement Disorder Society·P MartinelliB Barbiroli
Oct 4, 2000·The Journal of Experimental Medicine·L IziksonA D Luster
Dec 25, 2002·Neurobiology of Aging·Heiko BraakEva Braak
Jul 10, 2003·Lancet Neurology·Ya Ke, Zhong Ming Qian
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·John GreenwoodPeter Adamson
May 11, 2004·Movement Disorders : Official Journal of the Movement Disorder Society·Meltem MüftüogluNazmi Ozer
Aug 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Seonghan KimYoo-Hun Suh
Aug 18, 2004·Journal of Neurochemistry·Li-Ping Liang, Manisha Patel
Mar 26, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wei ZhangJing Zhang
Apr 6, 2005·The International Journal of Neuroscience·Hasmet Ayhan HanagasiMurat Emre
Jul 27, 2005·Trends in Neurosciences·Nadia StefanovaGregor K Wenning
Oct 13, 2005·Journal of the Neurological Sciences·Ahmad AgilFrancisco Vives
Oct 19, 2005·Brain : a Journal of Neurology·Don MahadRichard M Ransohoff
May 12, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Paula M KeeneyJames P Bennett
May 23, 2006·Lancet Neurology·Lonneke M L de Lau, Monique M B Breteler
Jul 29, 2008·Journal of Neuroimmunology·Stephanos KyrkanidesJohn A Olschowka
Feb 21, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Charlotte D'MelloMark G Swain
Mar 3, 2009·Neurobiology of Disease·Pier Giorgio MastroberardinoJ Timothy Greenamyre
Jun 1, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eun-Jung LeeHee-Sun Kim
Oct 21, 2010·BMC Neurology·Pilar del HoyoFélix Javier Jiménez-Jiménez
Nov 27, 2010·Cell Metabolism·Xiaolei QiuDanica Chen
May 23, 2012·Biochemical and Biophysical Research Communications·Aimei ZhangBo Yan

❮ Previous
Next ❯

Citations

Jun 26, 2018·Current Opinion in Clinical Nutrition and Metabolic Care·Mario OstSonia Moreno-Gomez

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
Assay

Software Mentioned

MedCalc
DatLab

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.