PMID: 403946May 11, 1977

Mitochondrial respiratory chain of Tetrahymena pyriformis. The thermodynamic and spectral properties

Biochimica Et Biophysica Acta
L Kilpatrick, M Erecińska

Abstract

The mitochondria isolated from the ciliate protozoon Tetrahymena pyriformis carry an oxidative phosphorylation with P/O ratio of 2 for succinate oxidation and P/O ratio of 3 for the oxidation of the NAD-linked substrates. The respiration is more than 90% inhibited with 1 mM cyanide while antimycin A and rotenone inhibit at concentrations of 1000-fold higher than those effective in mammalian mitochondria. Using a combination of spectral studies and potentiometric titrations, the components of the respiratory chain were identified and characterized with respect to the values of their half-reduction potentials. In the cytochrome bc1 region of the chain a cytochrome c was present with an Em7.2 of 0.225 V and two components with absorption maxima at 560 nm and the half-reduction potential values of -0.065 and -0.15 V at pH 7.2. The cytochrome with the more positive half-reduction potential was identified as the analogue of the cytochrome(s) b present in mitochondria of higher organisms, while the cytochrome with the more negative half-reduction potential was tentatively identified as cytochrome o. In addition ubiquinone was present at a concentration of approx. 4 nmol per mg mitochondrial protein. In the spectral region where cytoch...Continue Reading

References

Mar 1, 1975·Archives of Biochemistry and Biophysics·D F. Wilson, M Erecińska
May 30, 1973·Biochimica Et Biophysica Acta·H F Kauffman, B F van Gelder
Mar 1, 1971·Journal of General Microbiology·G TurnerB Chance
Aug 20, 1971·Biochemical and Biophysical Research Communications·J S Perlish, H J Eichel
Aug 1, 1973·Archives of Biochemistry and Biophysics·M ErecińskaB Chance
Nov 2, 1971·Biochimica Et Biophysica Acta·N SatoB Chance
Jun 24, 1971·FEBS Letters·N SatoB Chance
Sep 5, 1953·Nature·D KEILIN, J F RYLEY

Citations

Oct 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A GhelliM Degli Esposti
Mar 10, 1999·Chemosphere·M P SauvantE Piccinni
Jun 6, 2007·Doklady. Biochemistry and Biophysics·I V BrailovskayaE N Mokhova
Jul 1, 1979·Archives of Biochemistry and Biophysics·D F. WilsonI A Silver
Oct 1, 1979·Archives of Biochemistry and Biophysics·L Kilpatrick, M Erecińska
Dec 3, 1980·Biochimica Et Biophysica Acta·N V Katre, D F. Wilson
Aug 1, 1977·Archives of Biochemistry and Biophysics·D F. WilsonA Holian
Jul 1, 1990·Membrane Biochemistry·M BattinoM Marchetti

Related Concepts

Magnorbin
Carbon Monoxide
Isocyanides
Cytochromes
Sodium Dithionite
Udder
Mitochondria
Oxidative Phosphorylation
Oxygen Consumption
Columba livia

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