Oct 16, 1989

Mitochondrial rRNA-containing petite strains of yeast (Saccharomyces cerevisiae) show a normal nuclear-mitochondrial stringent response

Biochemical and Biophysical Research Communications
C M McEnteeA P Hudson

Abstract

The nuclear-mitochondrial stringent response was examined in isonuclear rho+, 21S rRNA-containing rho-, and rho o strains of S. cerevisiae. By 30 min after nutritional downshift, nuclear rDNA transcription falls to 15% of control levels congruently in all strains, as assayed via whole-cell RNA or by hybrid selection of specific double-labeled transcripts. Both in vivo and in vitro, the mitochondrial stringent response is identical between the rho- strain and its parental rho+ strain, and in both, the kinetics and magnitude of the organellar response mirror those of the nuclear response. The data show that mitochondrial transcription and protein synthesis are not required for stringent regulation of either nuclear or mitochondrial rDNA transcription.

Mentioned in this Paper

Stringent Response
RNA, mitochondrial
Saccharomyces cerevisiae allergenic extract
Transcription, Genetic
DNA, Ribosomal
RNA, Fungal
Protein Biosynthesis
Recombinant DNA
Mitochondria
21S rRNA

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