Mitochondrial Specific H2 S n Fluorogenic Probe for Live Cell Imaging by Rational Utilization of a Dual-Functional-Photocage Group

ACS Sensors
Linqi HanWei Huang

Abstract

Reactive sulfur species play a very important role in modulating neural signal transmission. Hydrogen polysulfides (H2S n, n > 1) are recently suggested to be the actual signaling molecules. There are still few spatiotemporal controllable-based probes to detect H2S n. In this work, for the first time, we proposed the photocleavage product of the common photoremovable protecting group (2-nitrophenyl moiety) capable of trapping H2S n. Taking advantage of this, we constructed the probe H1 containing a photocontrollable group, a mitochondrial directing unit and a signal reporter fluorescein dye. H1 exhibited excellent fluorescence enhancement (50-fold) in response to H2S n under the aqueous buffer only after UV irradiation. H1 also showed high selectivity and sensitivity for H2S n over other reactive sulfur species, reactive oxygen species, and other analytes, especially biothoils including hydrogen sulfide, cysteine, homocysteine, and glutathione. We showed the utility of H1 to image H2S n in living cells with high spatiotemporal resolution.

References

Jan 15, 1993·Science·A VermaS H Snyder
May 30, 2002·Biological Chemistry·Gregory I Giles, Claus Jacob
Nov 29, 2002·Journal of Cell Science·David S Bredt
Oct 18, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Yukiko KikuchiMizuo Maeda
Jul 12, 2012·Nature Reviews. Molecular Cell Biology·Bindu D Paul, Solomon H Snyder
Feb 16, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yuka KimuraHideo Kimura
Apr 16, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tomoaki IdaTakaaki Akaike
May 8, 2014·Antioxidants & Redox Signaling·Hideo Kimura
May 9, 2014·Journal of the American Chemical Society·Chunrong LiuMing Xian
Apr 8, 2015·Nature Reviews. Drug Discovery·John L Wallace, Rui Wang
Jun 18, 2015·Nature Chemical Biology·Tatiana V MishaninaRuma Banerjee
Dec 17, 2015·Journal of the American Chemical Society·Pramod K YadavRuma Banerjee
Dec 6, 2016·Angewandte Chemie·Bo PengShao Q Yao
May 27, 2017·Antioxidants·Gregory I GilesClaus Jacob
Aug 9, 2017·Organic & Biomolecular Chemistry·Neha GuptaManoj Kumar
Feb 1, 2018·Journal of the American Chemical Society·Jacob M GoldbergStephen J Lippard

❮ Previous
Next ❯

Citations

Jan 3, 2019·Chembiochem : a European Journal of Chemical Biology·Jia DongJingyan Ge
Jun 27, 2019·Analytical and Bioanalytical Chemistry·Xilang JinXianghan Zhang
Feb 5, 2021·Journal of Clinical Biochemistry and Nutrition·Honami Echizen, Kenjiro Hanaoka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.