Mitochondrial superoxide anion production and release into intermembrane space

Methods in Enzymology
Derick HanEnrique Cadenas

Abstract

The topological distribution of ubiquinone in the mitochondrial respiratory chain suggests that both ubiquinone pools may participate in O2.- production and, hence, are vectorially released into the matrix and intermembrane space. Mitoplasts, obtained by either digitonin or hypotonic KCl treatment, are a suitable experimental model for measuring O2.- in the intermembrane space. The use of membrane-impermeable spin-broadening agents strengthens the notion that part of the O2.- generated by the respiratory chain may be released into the intermembrane space. This, together with the putative occurrence of a Cu, Zn-superoxide dismutase in this compartment may account for part of H2O2 released by mitochondria and contributing to a cytosolic steady-state level of this species in cytosol.

Citations

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