Mitochondrial superoxide increases excitatory synaptic strength in spinal dorsal horn neurons of neuropathic mice

Molecular Pain
Chilman BaeJun-Ho La

Abstract

Reactive oxygen species has been suggested as a key player in neuropathic pain, causing central sensitization by changing synaptic strengths in spinal dorsal horn neurons. However, it remains unclear as to what type of reactive oxygen species changes what aspect of synaptic strengths for central sensitization in neuropathic pain conditions. In this study, we investigated whether mitochondrial superoxide affects both excitatory and inhibitory synaptic strengths in spinal dorsal horn neurons after peripheral nerve injury. Upregulation of mitochondrial superoxide level by knockout of superoxide dismutase-2 exacerbated neuropathic mechanical hypersensitivity caused by L5 spinal nerve ligation, whereas downregulation of mitochondrial superoxide level by overexpression of superoxide dismutase-2 alleviated the hypersensitivity. In spinal nerve ligation condition, the frequency of miniature excitatory postsynaptic currents increased, while that of miniature inhibitory postsynaptic currents decreased in spinal dorsal horn neurons. Superoxide dismutase-2-knockout augmented, whereas superoxide dismutase-2-overexpression prevented, the spinal nerve ligation-increased miniature excitatory postsynaptic currents frequency. However, superoxide...Continue Reading

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Citations

Nov 1, 2020·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Hee Kee KimSalahadin Abdi
Jun 26, 2020·Physiological Reviews·Nanna Brix FinnerupTroels Staehelin Jensen
Mar 29, 2021·Neuroscience Letters·Iryna A KhasabovaDonald A Simone
Nov 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Luísa Teixeira-SantosDora Pinho

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BETA
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SigmaPlot
pClamp
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Clampfit

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