PMID: 9550304Apr 29, 1998Paper

Mitochondrial transmembrane potential and free radical production in excitotoxic neurodegeneration

Naunyn-Schmiedeberg's Archives of Pharmacology
J H Prehn

Abstract

Excitotoxic cell death is involved in many forms of acute and chronic neurodegeneration. We induced excitotoxic cell death in cultured rat hippocampal neurons by brief exposure to two selective glutamate receptor agonists with different neurotoxic potencies, N-methyl-D-aspartate (NMDA) and kainate (KA). Digital video imaging was performed during exposure to the agonists to monitor free radical production and changes in mitochondrial transmembrane potential, psi(m). Brief exposure to NMDA (10 min) induced significant cell death in the hippocampal neurons reaching a maximum at a concentration of 300 microM (57.2+/-2.6% cell death; P<0.001). In parallel imaging experiments we found that exposure to NMDA (300 microM, 10 min) induced a significant increase in superoxide production monitored with the oxidation-sensitive probe, hydroethidine (increase of 280+/-33% above baseline; P<0.001). Rhodamine-123-based imaging revealed a loss of psi(m) in 70.1+/-10.1 % of the hippocampal neurons during the exposure to NMDA. In contrast to NMDA, brief exposure to KA (10 min) produced limited neurotoxicity reaching a maximum at a concentration of 100 microM (10.2+/-4.0% cell death; P<0.05). Exposure to KA (100 microM, 10 min) also caused a signif...Continue Reading

Citations

May 19, 2001·Annals of Neurology·K L Quick, L L Dugan
Dec 2, 2004·Journal of Neuroscience Research·P G SullivanJ E Springer
Oct 6, 2006·Naunyn-Schmiedeberg's Archives of Pharmacology·Dan ZhangJianjun Zhang
Aug 21, 2013·In Vitro Cellular & Developmental Biology. Animal·Ghazaleh S TayeboonS Nasser Ostad
Apr 15, 2004·Neurochemistry International·Y S KwonH C Kim
Jun 14, 2003·Pharmacology & Therapeutics·Jeffrey M Brown, Bryan K Yamamoto
Apr 27, 2000·Trends in Neurosciences·M E Rice
Sep 5, 2002·Free Radical Biology & Medicine·Doo Yeon KimByoung Joo Gwag
Feb 15, 2000·Annals of the New York Academy of Sciences·D G NichollsM W Ward
Mar 4, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N OmataY Yonekura
Feb 24, 2007·Archives of Biochemistry and Biophysics·Florian LangErich Gulbins
Dec 26, 2006·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Ana O FagundesEmilio L Streck
Nov 28, 2006·Neuroscience Research·Patrícia Fernanda SchuckMoacir Wajner
Jun 3, 2004·The Journal of Comparative Neurology·Patrick G SullivanStephen W Scheff
Jun 6, 2003·Journal of Neurochemistry·Duncan G MacGregorMargaret E Rice
Feb 19, 2005·Neurobiology of Aging·Hsueh-Meei HuangGary E Gibson
Jun 15, 2004·Neuroscience Letters·Yang-Hsin ShihYu-Show Fu
Feb 24, 2006·Brain Research·Márcio R MartinsJoão Quevedo
Jan 12, 2000·Experimental Neurology·P G SullivanS W Scheff
Oct 6, 2012·Journal of Molecular and Cellular Cardiology·Juha P NäpänkangasIlmo E Hassinen
Jun 8, 2007·Journal of Neurophysiology·Dominic P D'AgostinoJay B Dean
Apr 27, 2012·Physiological Reviews·Michelle L James, Sanjiv S Gambhir
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.