Mitochondrial transplantation by intra-arterial injection for acute kidney injury.

American Journal of Physiology. Renal Physiology
Ilias P DoulamisJames D McCully

Abstract

Acute kidney injury is a common clinical disorder and one of the major causes of morbidity and mortality in the postoperative period. In this study, the safety and efficacy of autologous mitochondrial transplantation by intra-arterial injection for renal protection in a swine model of bilateral renal ischemia-reperfusion injury were investigated. Female Yorkshire pigs underwent percutaneous bilateral temporary occlusion of the renal arteries with balloon catheters. Following 60 min of ischemia, the balloon catheters were deflated and animals received either autologous mitochondria suspended in vehicle or vehicle alone, delivered as a single bolus to the renal arteries. The injected mitochondria were rapidly taken up by the kidney and were distributed throughout the tubular epithelium of the cortex and medulla. There were no safety-related issues detected with mitochondrial transplantation. Following 24 h of reperfusion, estimated glomerular filtration rate and urine output were significantly increased while serum creatinine and blood urea nitrogen were significantly decreased in swine that received mitochondria compared with those that received vehicle. Gross anatomy, histopathological analysis, acute tubular necrosis scoring, ...Continue Reading

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Citations

Oct 13, 2020·Renal Failure·Balamurugan PackialakshmiXiaoming Zhou
Mar 7, 2021·International Journal of Molecular Sciences·Matthias Mietsch, Rabea Hinkel
Aug 27, 2021·Journal of Biochemical and Molecular Toxicology·Gokhan Burcin KubatSenay Akin

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Methods Mentioned

BETA
biopsy
Assay
transmission electron microscopy
electron microscopy

Software Mentioned

LabChart
STATA
GraphPad Prism

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