PMID: 9190205Feb 1, 1997Paper

Mitogen-activated protein kinase activation down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in G2-arrested oocytes

Molecular Biology of the Cell
A AbrieuA Picard

Abstract

The G2 arrest of oocytes from frogs, clams, and starfish requires that preformed cyclin B-cdc2 complexes [prematuration-promoting factor (MPF)] be kept in an inactive form that is largely due to inhibitory phosphorylation of this pre-MPF. We have investigated the role of mitogen-activated protein (MAP) kinase in the activation of this pre-MPF. The cytoplasm of both frog and starfish oocytes contains an activity that can rapidly inactivate injected MPF. When the MAP kinase of G2-arrested starfish or Xenopus oocytes was prematurely activated by microinjection of c-mos or Ste-11 delta N fusion proteins, the rate and extent of MPF inactivation was much reduced. Both effects were suppressed by expression of the specific MAP kinase phosphatase Pyst 1. These results show that MAP kinase down-regulates a mechanism that inactivates cyclin B-cdc2 kinase in Xenopus oocytes. In starfish oocytes, however, MAP kinase activation occurs only after germinal vesicle breakdown, much after MPF activation. In this case, down-regulation of the cyclin B-cdc2 inhibiting pathway is a sensitive response to hormonal stimulation that does not require MAP kinase activation.

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Citations

Sep 25, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J E Ferrell
Mar 11, 2000·Molecular Biology of the Cell·S A WalterJ E Ferrell
Sep 14, 2010·The Journal of Reproduction and Development·Ding-Xiao ZhangNam-Hyung Kim
Oct 30, 2004·Molecular Reproduction and Development·T L Smythe, S A Stricker
Sep 24, 1999·Developmental Biology·T Kishimoto
Mar 22, 2005·Biochimica Et Biophysica Acta·Edith Browaeys-PolyKatia Cailliau
Mar 1, 2005·Developmental Biology·Tomomi M YamamotoTakeo Kishimoto
Jun 7, 2005·Developmental Biology·Wen Ling ZhangBrigitte Ciapa
Aug 24, 2000·Molecular Reproduction and Development·N YoshidaM Yamashita
May 15, 2009·Biology of Reproduction·Se-Jin YoonKyung-Ah Lee
Jul 25, 2000·Biology of Reproduction·C de Vantéry ArrighiS Schorderet-Slatkine
May 31, 2007·Molecular Endocrinology·Cheng-Guang LiangQing-Yuan Sun
Mar 26, 2003·Genes & Development·Brian J Tunquist, James L Maller

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