Mitogen-activated protein kinase mediates purinergic-enhanced nerve growth factor-induced neurite outgrowth in PC12 cells

Journal of Neuroscience Research
Holger Peter Behrsing, P Richard Vulliet

Abstract

In 1999, we reported new observations that several compounds, including ATP, enhance neurite expression in PC12 cells when coapplied with nerve growth factor (NGF). Because purinergic and NGF signaling have several potential interfaces in PC12 cells, a series of experiments was conducted to elucidate the signal mediators contributing to the enhancement. Activities of selected kinases were measured and Western blots evaluated mitogen-activated protein kinase (MAPK) active and nonactive isoforms in lysates of the treated PC12 cells. In terms of purinergic potency, ATP and beta,gamma-methylene ATP elicited the greatest neurite-enhancing effect, whereas adenosine and alpha,beta-methylene ATP elicited the smallest. The effectiveness of a nonhydrolyzable analog such as beta,gamma-methylene ATP indicates that a nonmetabolic process is responsible. In response to ATP, NGF, or NGF + ATP, MAPK activity (measured by 32P incorporation) was maximal within 2 hr and remained statistically elevated over control levels throughout the 24 hr monitored. At maximal 32P incorporation, MAPK activity in response to ATP, NGF, and NGF + ATP was two-, four-, and sixfold higher, respectively, than control values; the observed increase was qualitatively co...Continue Reading

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Citations

Apr 28, 2006·Pflügers Archiv : European journal of physiology·Herbert Zimmermann
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·David B ArthurPaul A Insel
Feb 16, 2011·Seminars in Cell & Developmental Biology·Herbert Zimmermann
Aug 16, 2005·Molecular and Cellular Neurosciences·Charles D MillsClifford J Woolf
Aug 4, 2015·Neurochemical Research·Sophia L B OliveiraHenning Ulrich
Jul 12, 2011·Biochemical and Biophysical Research Communications·Masataka OdaJun Sakurai
Dec 20, 2011·The International Journal of Neuroscience·Lian-Feng LinHuan-Min Luo
Oct 27, 2015·Neuropharmacology·Claudia HeineHeike Franke

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