Mitogen-activated protein kinase/extracellular signal-regulated protein kinase activation of cultured human dental pulp cells by low-power gallium-aluminium-arsenic laser irradiation

International Endodontic Journal
H MiyataK Otsuka

Abstract

To examine whether low-power laser irradiation (LPLI) promotes cellular proliferation of human dental pulp-derived fibroblast-like cells (dental pulp cells). Dental pulp cells were obtained by primary culture of human dental pulp tissues from extracted third molar teeth. The phosphorylation of the mitogen-activated protein kinase (MAPK) family after LPLI of these cells was investigated by Western blotting. By using a specific MAPK/ERK kinase (MEK) inhibitor (PD098059), the specific effect of LPLI on the MAPK pathway was also investigated by Western blotting as described above. The incorporation of [3H]thymidine into the cells after LPLI was determined, and statistical analysis was performed by Wilcoxon signed-ranks test. Extracellular signal-regulated protein kinase (ERK) 1/2 was phosphorylated between 5 and 30 min after LPLI. Moreover, PD098059 inhibited LPLI-mediated ERK1/2 activation. LPLI did not affect p38 MAPK or c-Jun N-terminal kinase (JNK) phosphorylation. But LPLI did not stimulate [3H]thymidine incorporation into these cells. These results indicated that LPLI activated MAPK/ERK, a signal for proliferation, differentiation and survival, but did not activate the stress signals p38 MAPK and JNK in human dental pulp cells.

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Citations

Feb 27, 2010·Lasers in Medical Science·Verica AleksicYuichi Izumi
Aug 1, 2006·Photomedicine and Laser Surgery
Jul 30, 2010·Photomedicine and Laser Surgery·Philip V PeplowG David Baxter
Mar 2, 2012·Photomedicine and Laser Surgery·Luciana Batista PereiraKarina Fittipaldi Bombonato-Prado
Jul 22, 2011·Tissue Engineering. Part a·Akon HiguchiShih-Tien Hsu
Feb 15, 2014·Lasers in Medical Science·Águida Cristina Gomes HenriquesRoseana de Almeida Freitas
Sep 24, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Su Mi WooJi Yeon Jung
Feb 1, 2015·Journal of Endodontics·Young-Sun KwonKyung-San Min
Jun 26, 2013·World Journal of Methodology·Angela DomínguezOscar Mario Tamayo
Dec 15, 2006·World Journal of Gastroenterology : WJG·Parimal Chowdhury, Kodetthoor-B Udupa

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