PMID: 3529498Jan 1, 1986Paper

Mitogen-induced disorganization of capillary-like structures formed by human large vessel endothelial cells in vitro

Tissue & Cell
Allikmets EYu, S M Danilov

Abstract

Endothelial cells (EC) from human aorta, umbilical vein and pulmonary artery were grown in Medium 199 supplemented with 20% human serum (HS), endothelial cell growth factor (ECGF) from bovine and human brain (200 micrograms/ml) and heparin (100 micrograms/ml) in gelatin-coated flasks. Under these conditions cells rapidly proliferated and survived 15-25 passages (40-60 cumulative population doublings). When cells were cultured on plastic substrate and without growth factors a capillary-like network appeared after 3-4 weeks of growth. According to TEM, this network consisted of tubes with the lumen encircled by one or several cells. The reduction of serum concentration in the medium or the replacement of plasma-derived serum (PDS) for HS reduced the time of network formation to 3-5 days. S-180 conditioned medium mitogenic for EC induced a rapid spreading of the cells and a partial reversion to a two-dimensional monolayer structure. Trypsin inhibitor did not abolish the effect of tumour conditioned medium. Other EC mitogens, e.g. ECGF and fibroblast growth factor (FGF), also disorganized the capillary-like network. In a day or two the network was completely restored. In contrast, culturing EC on gelatin-coated substrate is a suffi...Continue Reading

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Citations

Jan 1, 1994·Biulleten' eksperimental'noĭ biologii i meditsiny·I V BaliasnikovaS M Danilov
Feb 1, 1994·Biulleten' eksperimental'noĭ biologii i meditsiny·T D CherkasovaV I Pokrovskiĭ
Feb 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D B Stolz, B S Jacobson
Mar 7, 2002·The Biochemical Journal·Irina V BalyasnikovaSergei M Danilov
Mar 1, 1988·Analytical Biochemistry·V R MuzykantovS M Danilov
Feb 1, 1990·Experimental Eye Research·J M Roberts, J V Forrester
Sep 17, 2004·Endothelium : Journal of Endothelial Cell Research·J DyeP Clark
Oct 1, 1988·British Journal of Pharmacology·P BregestovskiK Takeda
Jan 1, 1990·Fundamental & Clinical Pharmacology·V J RichardK A Reimer
Mar 1, 1991·Circulation·S W WernsB R Lucchesi
Jan 1, 1991·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C D AgardhB K Siesjö
Dec 25, 2003·The Journal of Physiology·Cristine E Berry, Joshua M Hare
Oct 27, 2006·European Journal of Heart Failure·Domingo A Pascual-FigalMariano Valdes
Jun 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katherine C WoodD Neil Granger
Sep 1, 1990·Circulation·R Bolli
Jul 1, 1995·Archives of Disease in Childhood. Fetal and Neonatal Edition·G A Russell, R W Cooke
Sep 1, 1999·Acta Physiologica Scandinavica·J M YoudM A Vincent
Sep 1, 1988·Experimental Eye Research·M Y PistsovS M Danilov

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