PMID: 6968815Nov 1, 1980Paper

Mitogen-initiated synthesis and secretion of T cell growth factor(s) by a T-lymphoma cell line

The Journal of Experimental Medicine
S ShimizuR T Smith

Abstract

Of 12 T-lymphoma cell lines investigated, one line, EL-4 azgr (Thy-1+, Lyt-1+, Lyt-2-3-,Ia-, T-200+, sIg-, and FcR-) and, to a lesser extent, the parental cell line, EL-4, produced T cell growth factor(s) (TCGF) when stimulated by the T-cell mitogen concanavalin A (Con A). Induced production of TCGF-E was detected by 6 h and maximal at 18-24 h. Purified TCGF-E from this source had an approximately 30,000 mol wt and the biological activity of TCGF produced by whole spleen cells, including: augmentation of T cell-mitogen responses, cytotoxic T lymphocyte (CTL) proliferation support dependence, augmented generation of CTL, lack of strain specificity, and failure to stimulate resting T cells. TCGF-E is neither synthesized or secreted by this lymphoma cell line unless stimulated by Con A. X-irradiation up to 7,000 rad failed to inhibit synthesis and secretion. These observations have a practical application in providing a relatively homogeneous clonal cell product for T cell culture support and for structural and functional studies of the TCGF molecule(s). They suggest also a model for examining mechanisms of triggering production and secretion of a regulatory molecule that controls T cell functions.

Citations

Aug 1, 1985·Biulleten' eksperimental'noĭ biologii i meditsiny·V V MalaĭtsevT M Andronova
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J S Lipsick, N O Kaplan
Nov 1, 1981·The Journal of Experimental Medicine·J E GootenbergR C Gallo
May 1, 1983·Experimental Cell Research·A RavidK H Stenzel

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