Mitogenic action of adenosine on osteoblast-like cells, MC3T3-E1

Calcified Tissue International
S Shimegi

Abstract

The purpose of this study was to investigate the mechanisms by which adenosine stimulates proliferation of osteoblast-like cells, MC3T3-E1. Adenosine by itself induces the stimulation of cell proliferation and accentuates the mitogenecity of PDGFs (AA and BB homodimers) for the cells. 8-Cyclopentyl-1,3-dimethylxanthine (CPX), a nonselective adenosine receptor antagonist, partially inhibited adenosine-induced DNA synthesis in a competitive manner, suggesting that the mitogenic action of adenosine is, at least in part, mediated by xanthine-sensitive receptors. In pertussis-toxin (PTX)-pretreated cells, adenosine- but not PDGF-BB-stimulated DNA synthesis was partially inhibited, and CPX did not exert a further inhibitory effect, suggesting an involvement of PTX-sensitive G-protein downstream of CPX-sensitive receptor. When adenosine uptake was prevented with dipyridamole, the stimulation of proliferation by adenosine was not decreased at all, indicating that the CPX-insensitive part of adenosine action is not associated with the uptake of adenosine and subsequent incorporation into the nucleotide pool. Adenosine did not influence the basal level or the PDGF-BB-induced increase in [Ca2+]i. Since it is known that the cAMP pathway ac...Continue Reading

Citations

Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
Jul 19, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·P FishmanA S Multani
Dec 6, 2008·Journal of Leukocyte Biology·Majid KatebiBruce N Cronstein
Jan 19, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Bronwen A J EvansJack Ham
Sep 2, 2011·Journal of Cellular Physiology·Masahide TakedachiShinya Murakami
Apr 24, 2019·Journal of Cellular Physiology·Matthew A VerasCheryle A Séguin
Jul 2, 2003·Cellular Signalling·Gunnar Schulte, Bertil B Fredholm

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