PMID: 8939780Dec 1, 1996Paper

Mitogenic action of hydrochlorothiazide on human osteoblasts in vitro: requirement for platelet-derived growth factor

Calcified Tissue International
K H LauJ E Wergedal

Abstract

Long-term use of hydrochlorothiazide (HCTZ), a common diuretic agent for hypertension, has been associated with increased bone density and reduced hip fracture rates in patients. In this study, we sought to examine whether HCTZ has an anabolic effect on the proliferation of human osteoblasts (derived from either vertebrate or rib bone samples) in vitro. Cell proliferation was determined by [3H]thymidine incorporation and cell number counting. In medium supplemented with 1% bovine calf serum, HCTZ significantly and reproducibly increased [3H]thymidine incorporation and cell number. The stimulatory effect was dose dependent in a biphasic manner, with the maximal stimulation (approximately 60% above control, P < 0.001) seen at 1 microM HCTZ. In fresh serum-free medium, HCTZ was ineffective as a bone cell mitogen, indicating that the bone cell mitogenic activity of HCTZ required a serum growth factor (GF). HCTZ at doses greater than 10 microM was inhibitory in the presence or the absence of serum, presumably because of the cytotoxic effects. The serum requirement for the bone cell mitogenic activity of HCTZ could be replaced with a conditioned medium (conditioned with normal human osteoblasts for 24 hours), or with a mitogenic dose...Continue Reading

References

Jan 16, 1991·JAMA : the Journal of the American Medical Association·D T FelsonD P Kiel
Feb 1, 1990·The New England Journal of Medicine·A Z LaCroixA M Ostfeld
Apr 1, 1989·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·T SteinicheF Melsen
Nov 28, 1986·Biochimica Et Biophysica Acta·J E WergedalD J Baylink
Jul 15, 1985·American Journal of Obstetrics and Gynecology·P Adland-DavenportJ F Pendergast
Feb 1, 1973·Metabolism: Clinical and Experimental·S MiddlerF C Bartter
May 1, 1984·Proceedings of the Society for Experimental Biology and Medicine·J E Wergedal, D J Baylink
Aug 11, 1983·The New England Journal of Medicine·R D WasnichJ M Vogel
Oct 1, 1994·Calcified Tissue International·T J Hall, M Schaueblin
Apr 1, 1993·Bone and Mineral·B Dawson-Hughes, S Harris
Jan 1, 1959·Scandinavian Journal of Clinical and Laboratory Investigation·B A LAMBERG, B KUHLBACK

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Citations

Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A LalandeM C De Vernejoul
Dec 3, 2010·Kidney International·Khashayar SakhaeeOrson W Moe
May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·R X-D SongR J Santen
Feb 13, 2003·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·D J Beevers

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