Mitogenic and protein synthetic activity of tissue repair cells: control by the postsurgical macrophage

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
M FukasawaG S diZerega

Abstract

It is well known that fibroblasts are a main source of extracellular matrix synthesis necessary for tissue repair. In addition, macrophages secrete products that are known to modulate synthesis of extracellular matrix. Accordingly, we studied the incorporation of [3H]thymidine, [3H]proline, and [35S]sulfate into macromolecules produced by fibroblasts recovered from the site of peritoneal tissue repair cultured with and without spent media from postsurgical peritoneal macrophages. Rabbits underwent resection and reanastomosis of their small intestines. Peritoneal exudative cells (PEC) were then collected on postsurgical day 5 and day 10 as well as from nonsurgical controls, separated by discontinuous Percoll gradient centrifugation, and cultured for 48 h. A second group of rabbits underwent peritoneal wall abrasion from which fibroblast tissue repair cells (TRC) were collected from the site of injury at postsurgical day 7 and maintained in culture for varying times. Incorporation of radiolabeled precursors into DNA, collagen, and sulfated proteoglycans was determined. Incorporation of [3H]thymidine and [3H]proline into untreated TRC gradually decreased with culture duration. Conversely, [35S]sulfate incorporation gradually incre...Continue Reading

References

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Citations

Jul 2, 2003·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·Tomoyuki ShirafujiHiroyoshi Ayabe
Apr 20, 2005·The Journal of Experimental Medicine·Julie M JamesonWendy L Havran
May 16, 1998·The Journal of Clinical Investigation·L A DiPietroR M Strieter
May 19, 2010·Journal of Cellular and Molecular Medicine·R CarmonaR Muñoz-Chápuli
Jul 1, 1990·The Journal of Surgical Research·M FukasawaG S diZerega
Nov 1, 1992·The Journal of Surgical Research·K E Rodgers, G S diZerega
Mar 1, 1996·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·A F Haney, E Doty
Jan 30, 2019·Nature Reviews. Nephrology·Patrick Ming-Kuen TangHui-Yao Lan
Jul 1, 1989·The Journal of Surgical Research·M FukasawaG S DiZerega

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