Mitogenic effect of gastrin and expression of gastrin receptors in duct-like cells of rat pancreas

Gastroenterology
I RoomanL Bouwens

Abstract

Ductular metaplastic cells are observed during pancreas injury. Growth control by gastrin and expression of gastrin/cholecystokinin (CCK) B receptors were evaluated in these cells. Acinoductal transdifferentiation was induced in vitro by culturing of acinar cells, and ductular metaplasia was obtained in vivo by ligation of the pancreatic ducts. Mitogenic effects of gastrin I on ductal complexes in vivo and of tetragastrin, pentagastrin, and gastrin I and II, with or without the CCK-B receptor antagonist L-365,260, on duct-like cells in vitro were analyzed by 5-bromo-2'-deoxyuridine labeling. Immunocytochemistry, Western blotting, and reverse-transcription polymerase chain reaction were applied for detection of the CCK-B receptor. Gastrin analogues induced a mitogenic stimulus in the duct-like cells in vitro and in ductal complexes in duct-ligated rat pancreas. Immunocytochemistry showed expression of CCK-B receptors in these models and in fetal but not normal adult exocrine pancreas. Additionally, up-regulation of CCK-B receptors during ductular metaplasia was shown by Western blotting and reverse-transcription polymerase chain reaction. Duct-like pancreatic epithelial cells in vitro and ductal complexes in vivo express gastrin...Continue Reading

References

Aug 12, 1976·Nature·L I LarssonR Håkanson
Nov 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L ScopsiL I Larsson
Mar 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L BouwensH Heimberg
Aug 1, 1993·European Journal of Biochemistry·S Silvente-PoirotD Fourmy
Jun 1, 1996·The Journal of Surgical Research·B J TsueiR H Bell
Apr 1, 1997·Acta Physiologica Scandinavica·H F HelanderJ H Walsh
May 16, 1998·Virchows Archiv : an International Journal of Pathology·Y Van NieuwenhoveG Willems
Jun 9, 1998·Gut·G S Baldwin, A Shulkes
Jul 29, 1998·International Journal of Cancer. Journal International Du Cancer·S A WatsonJ D Hardcastle
Sep 30, 1998·Gastroenterology·C Saillan-BarreauM Dufresne
Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·J MorissetJ Bourassa

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Citations

Oct 21, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jean MorissetJean Lainé
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jessy LardonLuc Bouwens
Dec 17, 2004·Pediatric Diabetes·Vijayakumar Ramiya, Desmond Schatz
Jan 31, 2008·American Journal of Physiology. Endocrinology and Metabolism·Thomas L JettonJack L Leahy
Feb 3, 2005·International Journal of Cancer. Journal International Du Cancer·Anne MathieuMarlène Dufresne
Jun 16, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Eugenio Bertelli, Moïse Bendayan
Jun 20, 2003·European Journal of Clinical Investigation·R Scharfmann
Oct 18, 2008·Diabetes, Obesity & Metabolism·L Baeyens, L Bouwens
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Oliver StrobelSarah P Thayer
Oct 13, 2009·Diabetes, Obesity & Metabolism·C DemetercoF Levine
Aug 7, 2012·Diabetes, Obesity & Metabolism·K FosgerauT Skovlund Ryge Neerup
Aug 14, 2012·Endocrine·Diana Boj-Carceller
Mar 1, 2012·Journal of Molecular Cell Biology·Grace E Asuelime, Yanhong Shi
Aug 16, 2017·Nature Reviews. Gastroenterology & Hepatology·Cara EllisTimothy J Kieffer
Sep 27, 2005·Physiological Reviews·Luc Bouwens, Ilse Rooman
Aug 4, 2004·Cell Biochemistry and Biophysics·Luc Bouwens
Apr 12, 2003·Pharmacology & Toxicology·Pascal ClercMarlène Dufresne
Jul 4, 2006·Physiological Reviews·Marlène DufresneDaniel Fourmy
Jan 18, 2006·Annual Review of Medicine·Laurie L Baggio, Daniel J Drucker
Apr 22, 2009·Journal of Cellular and Molecular Medicine·Kristen Brennand, Doug Melton
Aug 15, 2014·The Journal of Endocrinology·Noèlia Téllez, Eduard Montanya
Sep 2, 2014·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mallory E Bowers, Kerry J Ressler
Jul 9, 2005·Nature Biotechnology·Susan Bonner-Weir, Gordon C Weir
Sep 5, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maho KodamaGary Quinn
Sep 6, 2005·Differentiation; Research in Biological Diversity·Jessy Lardon, Luc Bouwens
Sep 28, 2006·The American Journal of Pathology·Ilse RoomanLuc Bouwens
Jan 20, 2007·Regulatory Peptides·Lennart Friis-Hansen

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