Mitogenic factors promoting intestinal smooth muscle cell proliferation

American Journal of Physiology. Cell Physiology
R D P StanzelM G Blennerhassett

Abstract

Intestinal smooth muscle cells are normally quiescent, but in the widely studied model of trinitrobenzene sulfonic acid (TNBS)-induced colitis in the rat, the onset of inflammation causes proliferation that leads to increased cell number and an altered phenotype. The factors that drive this are unclear and were studied in primary cultures of circular smooth muscle cells (CSMC) from the rat colon. While platelet-derived growth factor (PDGF)-AA, fibroblast growth factor (FGF), and epidermal growth factor (EGF) were ineffective, PDGF-BB and insulin-like growth factor-1 (IGF-1) caused significant increase in [(3)H]thymidine incorporation, bromodeoxyuridine uptake, and increased CSMC number, with PDGF-BB (≥0.2 nM) substantially more effective than IGF-1. Surprisingly, CSMC lacked expression of PDGF receptor-β (PDGF-Rβ) upon isolation but by 4 days in vitro, CSMC gained expression of PDGF-Rβ as shown by quantitative PCR, Western blot analysis, and immunocytochemistry; these CSMC responded to PDGF-BB but not IGF-1. PDGF-BB caused PDGF-Rβ phosphorylation and mobilization from the surface membrane, leading to activation of both Akt and ERK signaling pathways, which were essential for subsequent proliferation. In contrast, PDGF-AA, FGF, ...Continue Reading

References

Jun 1, 1992·The American Journal of Physiology·M G BlennerhassettS M Collins
Mar 11, 1997·The American Journal of Physiology·J M ZeehJ A McRoberts
Jul 9, 1998·The American Journal of Physiology·J F Kuemmerle, T L Bushman
Oct 9, 1999·The American Journal of Pathology·S SanovicM G Blennerhassett
Aug 29, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M G Blennerhassett, S Lourenssen
Nov 30, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·J B PucilowskaP K Lund
Apr 9, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E M ZimmermannJ B Pucilowska
Jun 28, 2001·Physiological Reviews·B C Berk
Aug 16, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·James G SimmonsP Kay Lund
Nov 1, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·John F KuemmerleJennifer G Bowers
Apr 3, 2004·Inflammatory Bowel Diseases·Gert Van AsschePaul Rutgeerts
Apr 8, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiping XinEllen M Zimmermann
Apr 27, 2004·Pflügers Archiv : European journal of physiology·R W Wells, M G Blennerhassett
Jun 15, 2004·Journal of the American College of Cardiology·Giuseppe G PietraR M Tuder
Jun 23, 2004·Cytokine & Growth Factor Reviews·James C Bonner
Jul 23, 2004·Physiological Reviews·Gary K OwensBrian R Wamhoff
Jul 2, 2005·Experimental and Molecular Pathology·Stacey L Marlow, Michael G Blennerhassett
Jul 30, 2005·The Journal of Comparative Neurology·Agueda LinMichael G Blennerhassett
Aug 16, 2005·Experimental Neurology·Sandra LourenssenMichael G Blennerhassett
Oct 1, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·John F Kuemmerle
Sep 8, 2006·American Journal of Physiology. Cell Physiology·Keiko Kawai-Kowase, Gary K Owens
Sep 8, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·J G SimmonsP K Lund
Jan 16, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M KurahashiS Torihashi
May 17, 2008·Genes & Development·Johanna AndraeChrister Betsholtz
Nov 26, 2008·Nature Reviews. Cancer·Michael Pollak
Apr 11, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Krystina B HazelgroveJohn F Kuemmerle
Jun 30, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jason H T BatesCharles G Irvin
Mar 27, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anne-Marie PelletierMichael G Blennerhassett

❮ Previous
Next ❯

Citations

Jan 7, 2015·Neurobiology of Disease·Shriram VenkataramanaM G Blennerhassett
Jan 15, 2014·Journal of Cellular and Molecular Medicine·Dileep G NairMichael G Blennerhassett
Apr 19, 2012·Journal of Tissue Engineering and Regenerative Medicine·Shivani SinghJames C Y Dunn
Dec 9, 2014·PloS One·Christopher M WalthersJames C Y Dunn
Oct 21, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B P HerringJ M Wo
Aug 16, 2018·American Journal of Physiology. Cell Physiology·Quinn A BonafigliaMichael G Blennerhassett
Aug 27, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Phyllissa Schmiedlin-RenEllen M Zimmermann
Feb 12, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dileep G NairMichael G Blennerhassett
Feb 6, 2021·Cellular and Molecular Neurobiology·M G Blennerhassett, S R Lourenssen
Jun 2, 2020·The American Journal of Pathology·Sandra R Lourenssen, Michael G Blennerhassett

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Gastrointestinal and Liver Physiology
Dileep G NairM G Blennerhassett
World Journal of Gastroenterology : WJG
Pinar AtacaOsman Yilmaz
American Journal of Physiology. Gastrointestinal and Liver Physiology
Anne-Marie PelletierM G Blennerhassett
© 2021 Meta ULC. All rights reserved