PMID: 6965791Jan 1, 1980Paper

Mitogenic hormone-induced intracellular message: assay and partial characterization of an activator of DNA replication induced by epidermal growth factor

Proceedings of the National Academy of Sciences of the United States of America
M Das

Abstract

This paper explores the pathway from nuclear quiescence to mitogenesis. It describes an in vitro assay for an activator of DNA replication induced by epidermal growth factor (EGF) in responsive cells. Cytoplasmic extracts from EGF-treated 3T3 cells were found to contain substances that can stimulate DNA synthesis in isolated nuclei from spleen cells of adult frogs. Extracts from untreated resting 3T3 cells lack this activity, and EGF itself is incapable of stimulating DNA synthesis in these cell-free systems. The extract-induced stimulation of incorporation of [3H]dTTP into nuclear DNA is ATP dependent and requires the presence of the four deoxyribonucleoside triphosphates, suggesting the occurrence of replication rather than repair synthesis. This cell-free assay has been used to obtain some initial insights into the mechanism of induction and biochemical characterization of the intermediate in EGF action. Half-maximal induction of the active intracellular substance is achieved at about 0.08 nM EGF, a concentration that correlates well with the concentration required for half-maximal mitogenesis. Studies on the biochemical characteristics of this active substance strongly suggest that the activity is associated with a protein....Continue Reading

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Citations

Jan 1, 1991·Journal of Cellular Biochemistry·F D CoffmanS Cohen
Sep 16, 1980·Biochemical and Biophysical Research Communications·S L LinM D Hollenberg
Jan 1, 1989·Experimental Gerontology·F D Coffman, S Cohen
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·J C Smith, C D Stiles
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M Das
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S BishayeeM Das
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R L WongS Cohen
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J K GutowskiS Cohen
Mar 1, 1982·Archives of Biochemistry and Biophysics·S Bishayee, M Das
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Jan 1, 1980·Journal of Supramolecular Structure·R W Holley
Jan 1, 1981·The Journal of Membrane Biology·M A Lieberman, L Glaser
Sep 1, 1982·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J St Hilaire, A L Jones
Jan 1, 1980·Journal of Supramolecular Structure·R A Bradshaw, J S Rubin
Feb 11, 1981·Molecular and Cellular Biochemistry·E D Adamson, A R Rees
Sep 1, 1980·Microbiological Reviews·S S DillsM H Saier

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