PMID: 9192871Jun 1, 1997Paper

Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity

Genes & Development
Janet VolkerU Hansen

Abstract

The mammalian transcription factor LSF (CP2/LBP-1c) binds cellular promoters modulated by cell growth signals. We demonstrate here that LSF-DNA-binding activity is strikingly regulated by induction of cell growth in human peripheral T lymphocytes. Within 15 min of mitogenic stimulation of these cells, the level of LSF-DNA-binding activity increased by a factor of five. The level of LSF protein in the nucleus remained constant throughout this interval. However, a rapid decrease in the electrophoretic mobility of LSF, attributable to phosphorylation, correlated with the increase in DNA-binding activity. pp44 (ERK1) phosphorylated LSF in vitro on the same residue that was phosphorylated in vivo, specifically at amino acid position 291, as indicated by mutant analysis. As direct verification of the causal relationship between phosphorylation and DNA-binding activity, treatment in vitro of LSF with phosphatase both increased the electrophoretic mobility of the protein and decreased LSF-DNA-binding activity. This modulation of LSF-DNA-binding activity as T cells progress from a resting to a replicating state reveals that LSF activity is regulated during cell growth and suggests that LSF regulates growth-responsive promoters.

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Citations

Sep 6, 2011·Journal of Molecular Histology·Renhua FanFeng Liu
Apr 27, 2004·Nucleic Acids Research·Hervé AcloqueJacques Samarut
Feb 7, 1998·Genes & Development·P L SheridanK A Jones
Jul 1, 2005·Molecular and Cellular Biology·Ho Chul KangChul Geun Kim
Aug 31, 2011·World Journal of Gastroenterology : WJG·Ren-Hua FanPing-Sheng Chen
Aug 24, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Fuyuhiko SatoKazuhiro Sogawa
Apr 14, 2006·International Journal of Immunogenetics·D McIlroyI Theodorou
Sep 30, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T MurataK Yasuda
Jun 21, 2014·Development·Valérie Nadeau, Jean Charron
Jul 15, 2003·Journal of Cellular Biochemistry·Zrinka PagonUlla Hansen
Aug 24, 1999·The Journal of Biological Chemistry·Z BingW S Liao
Sep 21, 2000·The Journal of Biological Chemistry·L RamamurthyS M Jane
Oct 28, 2015·Oncotarget·Yuji GotoMasashi Kato
Mar 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elise E DrouinUlla Hansen
May 3, 2012·Médecine sciences : M/S·Valérie NadeauJean Charron

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