PMID: 8593962Dec 1, 1995Paper

Mitomycin C induction of bacteriophages from Serpulina hyodysenteriae and Serpulina innocens

FEMS Microbiology Letters
Samuel B HumphreyN S Jensen

Abstract

A prophage was induced from cells of the pathogenic spirochaete Serpulina hyodysenteriae using mitomycin C. Five to seven hours after mitomycin C was added (8 micrograms/ml, final concentration) to S. hyodysenteriae B204 cultures in BHIS broth (OD620 = 0.9) cell lysis was detected as a decrease in culture optical density. Bacteriophage particles attached to whole cells and to cell debris were detected by electron microscopic analysis of negatively stained (2% PTA, pH 7.0) bacteria harvested by centrifugation from mitomycin C treated cultures. The phage particles consisted of a head (45 nm diameter) and a tail (64 nm x 9 nm). Bacteria from untreated cultures lacked phages detectable by electron microscopy. The appearance of bacteriophage particles in mitomycin C treated cultures correlated with the appearance of extrachromosomal DNA, 7-8 kb in size as estimated by agarose gel electrophoresis, in DNA preparations from treated S. hyodysenteriae cells. When cultures of other S. hyodysenteriae strains (B78, B169, A-1, B8044, B6933, Ack300/8, R-1) and S. innocens 4/71 in BHIS were treated with mitomycin C (8-15 micrograms/ml, final concentration), phages similar in morphology and size to the S. hyodysenteriae B204 were induced.

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Citations

Aug 2, 2003·FEMS Microbiology Letters·Thad B StantonRichard L Zuerner
Jun 12, 2012·Nature Reviews. Microbiology·Andrew S LangJ Thomas Beatty
Apr 25, 2001·Applied and Environmental Microbiology·T B StantonS B Humphrey
Jul 24, 2001·Journal of Bacteriology·C H EggersD S Samuels
Aug 20, 2005·Journal of Bacteriology·Eric G MatsonThad B Stanton
Dec 23, 2006·Trends in Microbiology·Andrew S Lang, J Thomas Beatty
Dec 21, 2013·Veterinary Microbiology·Luke J MappleyMartin J Woodward
Jul 15, 2005·Journal of Food Protection·James L Smith
Jun 30, 2019·Viruses·Dylan LawrenceScott A Handley
Aug 19, 2020·Viruses·Jonathan ColombetTélesphore Sime-Ngando
Aug 9, 2017·Annual Review of Virology·Andrew S LangJ Thomas Beatty

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