Mitotic block and delayed lethality in HeLa epithelial cells exposed to Escherichia coli BM2-1 producing cytotoxic necrotizing factor type 1.

Infection and Immunity
J De RyckeE Oswald

Abstract

The cytopathic effect (CPE) of Escherichia coli producing cytotoxic necrotizing factor type 1 (CNF1) was investigated by using a human epithelial cell (HeLa) model of infection with CNF1-producing E. coli BM2-1. This strain was shown to bind loosely, but massively, to HeLa cells. A 4-h interaction between bacteria and eukaryotic cells triggered the delayed appearance of a progressive dose-dependent CPE characterized by (i) intense swelling of cells accompanied by the formation of a dense network of actin stress fibers, (ii) inhibition of cell division due to a complete block in the G2 phase of the cell cycle, and (iii) nucleus swelling and chromatin fragmentation. These alterations resulted in cell death starting about 5 days after interaction. The absence of multinucleation clearly distinguished the CPE from the effect produced by cell-free culture supernatants of infected cells nor prevented by a CNF1-neutralizing antiserum. Pathogenicity was completely abolished after Tn5::phoA insertion mutagenesis in the cnf-1 structural gene but not restored by trans complementation with a recombinant plasmid containing intact cnf-1 and its promoter. These results suggest that a gene downstream of cnf-1, essential to the induction of the ...Continue Reading

References

Jul 9, 1992·Nature·A ZychlinskyP J Sansonetti
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Citations

Jun 23, 2006·Infection and Immunity·Loredana FalzanoCarla Fiorentini
Mar 22, 2018·MBio·Nadège Bossuet-GreifJean-Philippe Nougayrède
Jun 3, 2018·International Journal of Molecular Sciences·Elena TantilloMatteo Caleo
Jun 5, 2003·Veterinary Microbiology·Jacques G MainilEric Oswald
Feb 8, 2005·Current Opinion in Microbiology·Eric OswaldMotoyuki Sugai
Apr 25, 2000·European Journal of Cell Biology·J De RyckeC Tasca

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