Abstract
Chromosomal instability is common in bladder cancer and could be caused by mutations of mitotic checkpoint genes. Therefore we screened for mutations of the mitotic checkpoint genes hBUB1, hBUB1B, hBUB3 and TTK in six aneuploid bladder cancer cell lines and 15 human bladder tumours. The screening was performed by sequence analysis of the entire coding regions of the four genes. No mutations were detected in any of the four genes. We detected several sequence variations in hBUB1, hBUB1B and TTK both new and previously published. The genetic stability of the four gene loci were tested by loss of heterozygosity (LOH) analysis in the 15 patient samples, showing one LOH for each of the hBUB1B, hBUB3 and TTK loci (6.7%) of the cases, all in different tumour samples. No LOH was detected at hBUB1. We conclude that both mutational inactivation, and loss of one allele, of the examined mitotic checkpoint genes are relatively uncommon.
Citations
Apr 5, 2002·Cancer·Ayuko SaekiYuji Matsuzawa
Jan 5, 2013·Hormones & Cancer·Kiyoshi TakagiTakashi Suzuki
Oct 22, 2003·Archives of Medical Research·Carla S Lopes, Claudio E Sunkel
Oct 1, 2005·Nature Reviews. Cancer·Geert J P L KopsDon W Cleveland
Dec 24, 2010·Carcinogenesis·Naotaka NishiyamaYae Kanai
Oct 25, 2011·PloS One·Li BieMichael McClelland
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Yixin Yao, Wei Dai
Feb 4, 2014·Genome Medicine·Lisa EversMichael T Barrett
Apr 10, 2012·Annual Review of Biochemistry·Xuedong Liu, Mark Winey
Nov 21, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Pi-Chuan HsiehSheng-Fung Lin
Aug 19, 2008·Biochimica Et Biophysica Acta·Floris FoijerPeter K Sorger
Mar 14, 2007·Cancer Genetics and Cytogenetics·Yoshiaki YamamotoKohsuke Sasaki
Apr 15, 2003·The Journal of Pathology·Heike GrabschWolfram Mueller
Apr 17, 2008·Molecular Systems Biology·Sujun HuaKevin P White
Nov 12, 2010·Head & Neck·Clara RizzardiMauro Melato
Aug 5, 2005·International Journal of Cancer. Journal International Du Cancer·Emma CamachoLuis Hernández
Nov 18, 2003·Annual Review of Genetics·Daniel J Lew, Daniel J Burke
Jun 29, 2010·Cancer Biology & Therapy·Hannah CarterRachel Karchin
May 14, 2016·Japanese Journal of Clinical Oncology·Yoshiaki YamamotoHideyasu Matsuyama
Nov 21, 2014·PloS One·Fung ZhaoAnnie N Y Cheung
Jul 16, 2009·British Journal of Cancer·Y-K LeeH Lee
Jun 19, 2003·Oncogene·Michal Oskar NowickiTomasz Skorski
Feb 10, 2018·Oncotarget·Jia WangMaode Wang
Jan 27, 2004·The Journal of Pathology·Heike I GrabschWolfram Mueller
Apr 9, 2021·BioMed Research International·Annapurna S DSmita C Pawar