Mitotic chromosome assembly despite nucleosome depletion in Xenopus egg extracts

Science
Keishi ShintomiTatsuya Hirano

Abstract

The nucleosome is the fundamental structural unit of eukaryotic chromatin. During mitosis, duplicated nucleosome fibers are organized into a pair of rod-shaped structures (chromatids) within a mitotic chromosome. However, it remains unclear whether nucleosome assembly is indeed an essential prerequisite for mitotic chromosome assembly. We combined mouse sperm nuclei and Xenopus cell-free egg extracts depleted of the histone chaperone Asf1 and found that chromatid-like structures could be assembled even in the near absence of nucleosomes. The resultant "nucleosome-depleted" chromatids contained discrete central axes positive for condensins, although they were more fragile than normal nucleosome-containing chromatids. Combinatorial depletion experiments underscored the central importance of condensins in mitotic chromosome assembly, which sheds light on their functional cross-talk with nucleosomes in this process.

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Citations

Jun 24, 2017·Science·Yasutaka Kakui, Frank Uhlmann
Sep 25, 2017·Current Genetics·Yasutaka Kakui, Frank Uhlmann
Jan 20, 2018·Science·Johan H GibcusJob Dekker
Aug 25, 2017·Molecular Biology of the Cell·Takao OnoTatsuya Hirano
May 8, 2018·The Journal of Cell Biology·Christoph SchiklenkChristian H Haering
Nov 10, 2017·Cold Spring Harbor Symposia on Quantitative Biology·Keishi Shintomi, Tatsuya Hirano
Sep 30, 2018·Molecular & Cellular Proteomics : MCP·Shinya OhtaJuri Rappsilber
Sep 28, 2017·Nature Genetics·Tatsuya Hirano
Sep 25, 2019·Proceedings of the National Academy of Sciences of the United States of America·Hugo B BrandãoLeonid A Mirny
Oct 3, 2019·Annual Review of Genetics·Stanislau YatskevichKim Nasmyth
Nov 4, 2017·Science·Kim Nasmyth
Dec 10, 2019·The Plant Journal : for Cell and Molecular Biology·Hefei WangFangpu Han
Oct 17, 2017·Plant Cell Reports·Stefanie Dukowic-SchulzeChangbin Chen
Apr 7, 2020·ELife·Edward J BaniganLeonid A Mirny
Jun 21, 2018·Current Protocols in Cell Biology·Keishi Shintomi, Tatsuya Hirano
Nov 8, 2018·Nature·Matthew Good, Xavier Trepat
Dec 20, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Katerina V Gurova
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Feb 1, 2019·Molecular & Cellular Proteomics : MCP·Shinya OhtaJuri Rappsilber
Apr 11, 2021·Seminars in Cell & Developmental Biology·James R PaulsonWilliam C Earnshaw
May 20, 2021·Nature Communications·Keishi Shintomi, Tatsuya Hirano
Jun 15, 2021·Physical Biology·Joan-Ramon Daban
Dec 22, 2020·ACS Nano·Anthony Birnie, Cees Dekker

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