Mitotic recombination between homologous chromosomes drives genomic diversity in diatoms.

Current Biology : CB
Petra BulankovaLieven De Veylder

Abstract

Diatoms, an evolutionarily successful group of microalgae, display high levels of intraspecific genetic variability in natural populations. However, the contribution of various mechanisms generating such diversity is unknown. Here we estimated the genetic micro-diversity within a natural diatom population and mapped the genomic changes arising within clonally propagated diatom cell cultures. Through quantification of haplotype diversity by next-generation sequencing and amplicon re-sequencing of selected loci, we documented a rapid accumulation of multiple haplotypes accompanied by the appearance of novel protein variants in cell cultures initiated from a single founder cell. Comparison of the genomic changes between mother and daughter cells revealed copy number variation and copy-neutral loss of heterozygosity leading to the fixation of alleles within individual daughter cells. The loss of heterozygosity can be accomplished by recombination between homologous chromosomes. To test this hypothesis, we established an endogenous readout system and estimated that the frequency of interhomolog mitotic recombination was under standard growth conditions 4.2 events per 100 cell divisions. This frequency is increased under environmenta...Continue Reading

References

Nov 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·M F Abdullah, R H Borts
Feb 12, 2002·Nature Genetics·Jan M LuchtBarbara Hohn
Jun 23, 2006·Nature Methods·Richard WilliamsAndrew D Griffiths
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Maria A Barbera, Thomas D Petes
Mar 3, 2007·Current Biology : CB·Michael M DesaiAndrew W Murray
May 1, 2007·PLoS Genetics·Sijmen E SchoustraRolf F Hoekstra
Sep 13, 2007·Nature Reviews. Molecular Cell Biology·Benoît D'Autréaux, Michel B Toledano
Nov 15, 2007·The Journal of Biological Chemistry·Michael R Lieber
Jun 7, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Victor A ChepurnovWim Vyverman
Oct 17, 2008·Nature·Chris BowlerIgor V Grigoriev
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
May 7, 2011·Genetics·Gregory I LangMichael M Desai
Aug 5, 2011·Environmental Science & Technology·Tore BrembuAtle M Bones
Nov 9, 2014·Genetics·Lorraine S SymingtonMichael Lisby
Jan 1, 2014·Scientific Data·Kristi E KimJane M Landolin
Jun 2, 2015·Scientific Data·Stéphane PesantUNKNOWN Tara Oceans Consortium Coordinators
Oct 30, 2015·Cold Spring Harbor Perspectives in Biology·Neil Hunter
Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Shruti MalviyaChris Bowler
Apr 16, 2016·Nature Communications·Ivan SovićNiranjan Nagarajan
Jun 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Luis ZapataKorbinian Schneeberger
Oct 16, 2016·Nature Reviews. Genetics·Michael LynchPatricia L Foster
Dec 4, 2014·Biomolecular Detection and Quantification·Elena KalleChristopher Rensing
Jan 17, 2017·Nature·Thomas MockIgor V Grigoriev
Jul 19, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anna Godhe, Tatiana Rynearson
Sep 25, 2017·Genome Biology·Christopher QuinceA Murat Eren
Nov 8, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Laurie S StevisonRita M Graze

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