Mitral valvular interstitial cell responses to substrate stiffness depend on age and anatomic region.

Acta Biomaterialia
Elizabeth H StephensK Jane Grande-Allen

Abstract

The material properties of heart valves depend on the subject's age, the state of the disease and the complex valvular microarchitecture. Furthermore, valvular interstitial cells (VICs) are mechanosensitive, and their synthesis of extracellular matrix not only determines the valve's material properties but also provides an adhesive substrate for VICs. However, the interrelationship between substrate stiffness and VIC phenotype and synthetic properties is poorly understood. Given that the local mechanical environment (substrate stiffness) surrounding VICs differs among different age groups and different anatomic regions of the valve, it was hypothesized that there may be an age- and valve-region-specific response of VICs to substrate stiffness. Therefore, 6-week-, 6-month- and 6-year-old porcine VICs from the center of the mitral valve anterior leaflet (MVAC) and posterior leaflet (PML) were seeded onto poly(ethylene) glycol hydrogels of different stiffnesses and stained for markers of VIC activation (smooth muscle alpha-actin (SMaA)) and collagen synthesis (heat shock protein-47 (HSP47), prolyl 4-hydroxylase (P4H)). Six-week-old MVAC demonstrated decreased SMaA, P4H and HSP47 on stiffer gels, while 6-week-old PML only demonstra...Continue Reading

References

Dec 1, 1991·Journal of Biomedical Materials Research·W R GombotzA S Hoffman
Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Dec 4, 2003·Biomaterials·Xiao Zheng ShuGlenn D Prestwich
Sep 16, 2004·Journal of Biomedical Materials Research. Part a·Kristyn S MastersKristi S Anseth
Dec 2, 2004·Cell Motility and the Cytoskeleton·Tony YeungPaul A Janmey
Sep 13, 2005·The Journal of Thoracic and Cardiovascular Surgery·K Jane Grande-AllenPatrick M McCarthy
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Aug 29, 2006·Experimental Cell Research·Jiang WuJürgen Brinckmann
Sep 26, 2006·The Annals of Thoracic Surgery·Michael S SacksJoseph H Gorman
Oct 13, 2006·Annals of Biomedical Engineering·Kartik BalachandranAjit P Yoganathan
Dec 30, 2006·Circulation·Wayne RosamondUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
May 4, 2007·Biomaterials·Melinda C CushingKristi S Anseth
Nov 30, 2007·Biophysical Journal·Jérôme SolonPaul A Janmey
Dec 19, 2007·Circulation·Wayne RosamondUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
Feb 12, 2008·American Journal of Physiology. Heart and Circulatory Physiology·M PhoC A McCulloch
Feb 4, 2009·Journal of Biomedical Materials Research. Part a·Elizabeth H StephensK Jane Grande-Allen
Jun 19, 2009·Journal of Biomechanics·Gaurav KrishnamurthyNeil B Ingels
Oct 10, 2009·Tissue Engineering. Part a·Elizabeth H StephensK Jane Grande-Allen
Oct 10, 2009·Annals of Biomedical Engineering·Stephanie Nemir, Jennifer L West

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Citations

Jan 12, 2011·Current Cardiology Reports·K Jane Grande-Allen, Jun Liao
Feb 3, 2016·Basic Research in Cardiology·Cristiano SpadaccioAlberto Rainer
Feb 2, 2011·Advanced Drug Delivery Reviews·Jonathan T ButcherLaura A Hockaday
Dec 4, 2015·Journal of Tissue Engineering and Regenerative Medicine·Yan WuJennifer L West
Sep 28, 2012·Journal of Biomedical Materials Research. Part a·Bin DuanJonathan T Butcher
Sep 8, 2015·Biomechanics and Modeling in Mechanobiology·Tao Jin, Ilinca Stanciulescu
Dec 10, 2014·Annals of Biomedical Engineering·Arash KheradvarSuncica Canic
Apr 24, 2016·Annals of Biomedical Engineering·Laura Hockaday KangJonathan Talbot Butcher
Feb 11, 2015·Microvascular Research·C Alexander ArevalosK Jane Grande-Allen
Sep 10, 2016·Tissue Engineering. Part a·Christopher DebordeAgneta Simionescu
Dec 20, 2020·Journal of Cardiovascular Development and Disease·Karthik M KodigepalliJoy Lincoln
Feb 3, 2021·Cardiovascular Engineering and Technology·Daniel P Howsmon, Michael S Sacks
Oct 22, 2016·Journal of Biomechanics·Ishita TandonKartik Balachandran

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