Mixed Aetiology of Diarrhoea in Infants Attending Clinics in the North-West Province of South Africa: Potential for Sub-Optimal Treatment

Pathogens
Martina O ChukwuC L Obi

Abstract

Routine diagnostic methods for the aetiologic agents of diarrhoea in most developing countries are usually not sensitive enough, leading to under-diagnosis. Thus, this study investigated possible mixed diarrhoeal aetiology by using cultures and real-time polymerase chain reactions (PCR) in children younger than four years old in the Northwest Province, South Africa. In total, 505 stool samples were collected from symptomatic and asymptomatic children who were attending three clinics and the Brits hospital in Madibeng District, between September 2016 and December 2017. Rotavirus, norovirus, Campylobacter, Arcobacter, and diarrhoeagenic Escherichia coli (DEC) were targeted. Campylobacter spp. (24.6%), Arcobacter (15.8%) and DEC (19.6%) were detected using PCR; only Campylobacter spp. (29.7%) and DEC (26.9%) were detected through the culture. Campylobacterjejuni (36%), Campylobactercoli (28%), Campylobacterupsalensis (12%), and Arcobacterbutzleri (15.8%) were the only spp. of Campylobacter and Arcobacter identified. The eaeA gene (31.4%) of enteropathogenic E. coli/enterohaemorrhagic E. coli (EPEC/EHEC) was the most prevalent DEC virulence gene (VG) identified. Rotavirus and norovirus were detected at 23.4% and 20%, respectively. ...Continue Reading

References

Nov 8, 2003·Pediatric Research·Ellen CrushellBilly Bourke
Dec 6, 2003·The Journal of Pediatrics·Rocío MartínJuan M Rodríguez
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Apr 20, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Cindy R FriedmanUNKNOWN Emerging Infections Program FoodNet Working Group
Jun 18, 2004·Emerging Infectious Diseases·Steen EthelbergKåre Mølbak
Sep 15, 2004·FEMS Immunology and Medical Microbiology·Laila F NimriRaymond Batchoun
Apr 15, 2005·Emerging Infectious Diseases·Richard M LynnGeraldine A Willshaw
Jun 21, 2005·Microbiology and Immunology·Keiko KimataYoshiyuki Nagai
Nov 7, 2006·Journal of Animal Science·C L Gyles
Jul 5, 2007·Journal of Virology·J Joukje SiebengaMarion Koopmans
Nov 10, 2007·Memórias do Instituto Oswaldo Cruz·Vanessa BuerisLuiz Rachid Trabulsi
May 19, 2009·Marine Environmental Research·Damian Fernandez-JoverFrancisco Javier Martinez Lopez
Sep 29, 2009·Journal of the American Dietetic Association·Elizabeth Metallinos-KatsarasJan Kallio
Dec 24, 2009·Journal of Medical Virology·Kirsten MattisonSabah Bidawid
Jan 12, 2010·Journal of Infection in Developing Countries·Iruka N Okeke
Sep 28, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Janet MansMaureen B Taylor
Nov 4, 2010·Journal of Infection in Developing Countries·Mayar M SaidMohamed S Salama
Nov 9, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C PaschkeK-H Herbinger
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Jan 7, 2011·Journal of Clinical Microbiology·Emilie BessèdeFrancis Mégraud
Mar 18, 2011·The Pediatric Infectious Disease Journal·Chhour Y MengCarl J Mason
Aug 13, 2011·Journal of Medical Virology·Mohamed AbugaliaNigel Cunliffe
Aug 23, 2011·Current Opinion in Infectious Diseases·Theresa J Ochoa, Carmen A Contreras
Dec 16, 2011·Journal of Clinical Microbiology·Laid DouidahKurt Houf
Sep 4, 2012·Vaccine·L Mapaseka SeheriA Duncan Steele
Nov 24, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A P HeikemaW J B van Wamel
Feb 6, 2013·PLoS Neglected Tropical Diseases·Gwenyth LeeMargaret Kosek
Mar 23, 2013·Journal of Perinatology : Official Journal of the California Perinatal Association·J A KohlerW T Bass
Apr 16, 2013·Lancet·Christa L Fischer WalkerRobert E Black
Apr 26, 2013·Archives of Virology·Annelies KronemanMarion Koopmans
May 8, 2013·Journal of Applied Microbiology·G KaradasG Gölz
Jun 15, 2013·Emerging Infectious Diseases·Piyali MukherjeeAsish K Mukhopadhyay
Jul 6, 2013·Journal of Microbiological Methods·Beilei GePatrick F McDermott
Sep 18, 2013·The Journal of Infectious Diseases·Mami TaniuchiEric R Houpt
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
Oct 15, 2013·Journal of Medical Virology·Janet MansMaureen B Taylor

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS ( Statistical Package for the Social Sciences
Rotor
[UNK] real - time

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