Abstract
Recent studies evaluated the impact of dust exposure on pure and mixed cultures of Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, and Pseudomonas aeruginosa, revealing increased biofilm formation and altered sensitivities to H2O2. In this study, we examined the impact of lead (Pb), house, road, and combined dust on K. pneumoniae and P. aeruginosa in pure, mixed, or eukaryotic co-culture with human alveolar basal epithelial (A549) cells. Although no impact on pure or mixed culture growth was observed when bacteria were exposed to Pb, house, or road dust, increased biofilm was produced by P. aeruginosa in the presence of 0.8 μg/mL of Pb, while P. aeruginosa and K. pneumoniae both exhibited increased biofilm production in the presence of 100 μg/mL of house, road, and combined dust. When co-cultured with eukaryotic A549 cells, both bacteria demonstrated increased proliferation 6 h post-infection when challenged with house, road, or combined dust. However, when mixed bacteria were co-cultured with A549 cells, P. aeruginosa exhibited a significant ~ 1.5-fold increased proliferation in the presence of 100 μg/mL house, road, or combined dust. In sharp contrast, K. pneumoniae exhibited significantly reduced proliferatio...Continue Reading
References
Jan 1, 1992·Infection and Immunity·A Darfeuille-MichaudB Joly
Jun 1, 1992·Infection and Immunity·S MerinoJ M Tomás
Mar 1, 1991·Infection and Immunity·E ChiS Lory
Sep 1, 1991·Infection and Immunity·N R BakerD E Woods
May 1, 1991·Journal of Bacteriology·M J Gambello, B H Iglewski
Jan 1, 1990·Infection and Immunity·P DoigR T Irvin
Dec 1, 1990·The Journal of Clinical Investigation·T J StandifordR M Strieter
Dec 1, 1986·Infection and Immunity·X Nassif, P J Sansonetti
Jan 1, 1985·Infection and Immunity·R Ramphal, G B Pier
Oct 1, 1983·Infection and Immunity·R E HancockG B Pier
May 1, 1994·The Journal of Experimental Medicine·D R SmithR M Strieter
Apr 1, 1993·Infection and Immunity·M J GambelloB H Iglewski
Jul 1, 1997·Infection and Immunity·T A Oelschlaeger, B D Tall
Mar 20, 2003·Journal of Bacteriology·Martin SchusterE P Greenberg
Aug 1, 1959·Journal of General Microbiology·J P DUGUID
Aug 1, 1959·Postgraduate Medical Journal·K M PERRY
May 12, 2005·The Science of the Total Environment·Han YongmingEric S Posmentier
Mar 17, 2007·The Journal of Biological Chemistry·Matthew J BottomleyAndrea Carfì
Mar 15, 2008·Nature Reviews. Microbiology·Aras KadiogluPeter W Andrew
Jun 3, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ece Karatan, Paula Watnick
Oct 13, 2010·Journal of Environmental Management·Trang T T Duong, Byeong-Kyu Lee
Apr 25, 2012·PloS One·Denina HospodskyJordan Peccia
Apr 23, 2014·American Journal of Respiratory and Critical Care Medicine·Daniel R NeillAras Kadioglu
Sep 14, 2015·The Science of the Total Environment·Mohammed O SurajuJason A Rosenzweig
Nov 8, 2016·Chest·Patrick D Mitchell, Paul M O'Byrne
May 4, 2017·PloS One·Ejovwoke F DosunmuVida A Dennis
Jul 26, 2017·Scientific Reports·Anna Lena JungBernd Schmeck
Aug 5, 2017·Applied Microbiology and Biotechnology·Mariam BadoJason A Rosenzweig
Oct 19, 2017·Molecular Medicine Reports·Shan ShiHaibo Li
May 17, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinjing Guo, Ying Cheng
Jun 8, 2018·The Analyst·Oluwasola LawalStephen J Fowler