Mixed bacterial responses to dust exposure in an A549 eukaryotic co-culture

Applied Microbiology and Biotechnology
Mariam BadoJason A Rosenzweig

Abstract

Recent studies evaluated the impact of dust exposure on pure and mixed cultures of Escherichia coli, Enterococcus faecalis, Klebsiella pneumoniae, and Pseudomonas aeruginosa, revealing increased biofilm formation and altered sensitivities to H2O2. In this study, we examined the impact of lead (Pb), house, road, and combined dust on K. pneumoniae and P. aeruginosa in pure, mixed, or eukaryotic co-culture with human alveolar basal epithelial (A549) cells. Although no impact on pure or mixed culture growth was observed when bacteria were exposed to Pb, house, or road dust, increased biofilm was produced by P. aeruginosa in the presence of 0.8 μg/mL of Pb, while P. aeruginosa and K. pneumoniae both exhibited increased biofilm production in the presence of 100 μg/mL of house, road, and combined dust. When co-cultured with eukaryotic A549 cells, both bacteria demonstrated increased proliferation 6 h post-infection when challenged with house, road, or combined dust. However, when mixed bacteria were co-cultured with A549 cells, P. aeruginosa exhibited a significant ~ 1.5-fold increased proliferation in the presence of 100 μg/mL house, road, or combined dust. In sharp contrast, K. pneumoniae exhibited significantly reduced proliferatio...Continue Reading

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Methods Mentioned

BETA
Protein Assay
electrophoresis
ELISA
ELISArray

Software Mentioned

Graph Pad Prism
Odyssey

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