Mixed peptide-chitosan membranes to mimic the biological activities of a multifunctional laminin alpha1 chain LG4 module

Biomaterials
Kentaro HozumiMotoyoshi Nomizu

Abstract

Laminin alpha1 chain LG4 module is multifunctional and interacts with syndecans and integrin alpha2beta1 via AG73 (RKRLQVQLSIRT) and EF-I (DYATLQLQEGRLHFMFDLG) sites, respectively. Here, we conjugated the AG73 and EF1zz (ATLQLQEGRLHFXFDLGKGR, X: Nle) peptides on a chitosan membrane in various ratios to develop an LG4 mimic biomaterial. The AG73-chitosan membrane promoted strong cell attachment with membrane ruffling and the EF1zz-chitosan membrane promoted integrin-mediated cell adhesion with well-organized actin stress fibers. When AG73 and EF1zz were conjugated on a chitosan membrane with 1:9 molar ratio, the mixed peptide-chitosan membrane promoted the strong cell attachment and neurite outgrowth similar to that on the recombinant LG4 protein. Well-organized actin stress fibers and vinculin accumulated focal contacts were observed in the cells attached on the AG73:EF1zz (molar ratio=1:9)-chitosan membrane. These results suggest that the mixed peptide-chitosan membrane interacts with both syndecans and integrin alpha2beta1 and mimics the cell adhesion of a multifunctional LG4 protein. The mixed peptide-chitosan approach has potential as a multifunctional biomaterial for cell and tissue engineering.

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Citations

Jul 6, 2010·FEBS Letters·Kentaro HozumiMotoyoshi Nomizu
Mar 23, 2011·Biomaterials·Yuji YamadaMotoyoshi Nomizu
Aug 24, 2010·Archives of Biochemistry and Biophysics·Kentaro HozumiMotoyoshi Nomizu
Oct 6, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Nobuharu SuzukiMotoyoshi Nomizu
Oct 18, 2011·Chemistry : a European Journal·Yuji YamadaMotoyoshi Nomizu
Oct 7, 2011·Journal of Biomedical Materials Research. Part a·Yamato KikkawaMotoyoshi Nomizu
Jun 22, 2010·Biopolymers·Yuji YamadaMotoyoshi Nomizu
Mar 7, 2012·Archives of Biochemistry and Biophysics·Fumihiko KatagiriMotoyoshi Nomizu
Mar 10, 2017·Journal of Biomaterials Science. Polymer Edition·Daniel E Heath, Stuart L Cooper
Aug 21, 2018·Current Protocols in Cell Biology·Kentaro Hozumi, Motoyoshi Nomizu
Sep 14, 2018·International Journal of Molecular Sciences·Kentaro Hozumi, Motoyoshi Nomizu
Jan 15, 2019·Journal of Biomaterials Applications·Kentaro HozumiMotoyoshi Nomizu
Oct 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Li-Mei WangJian Ji
Dec 9, 2020·Biomacromolecules·Kamakshi BankotiSantanu Dhara
Dec 29, 2020·Experimental Cell Research·Hideki HayashiMotoyoshi Nomizu
Mar 4, 2021·Bioconjugate Chemistry·Neeraj KulkarniBichismita Sahu

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