Abstract
Mixed-phenotype acute leukemia (MPAL) is the infrequent type of acute leukemia characterized by immunophenotypic and/or cytochemical features of both lineages, but the diagnosis of this disease still is a challenge. In this study, we analyzed immunophenotyping, cytochemistry and frequency of MPAL patients to better diagnosis of MPAL characteristics according to WHO 2016 criteria for the first time in Iran. In this retrospective study, 27 patients were diagnosed as MPAL based on WHO 2016 criteria during 2014-2017. Flow cytometric immunophenotyping was performed on PB and BM samples evaluation of different CD marker expressions in MPAL subsets. RT-PCR was performed for the analyses of BCR/ABL1 fusion in MPAL subsets. Among 27 cases, (70.4%) 19 cases were B + My, (22.22%) 6 cases were T + My, and 2 cases (7.40%) were B + T + My. CD34, CD19, HLA-DR, TdT, CD22, iMPO were positive in majority of B + My cases. CD45, iMPO, iCD3, CD7, CD2 and CD5 were positive in majority of T + My cases. HLA-DR, TdT, CD10, CD22, iCD79a, iMPO, CD45, iCD3, CD7, CD3, CD2, CD5 were positive in majority of B + T + My cases. BCR/ABL1 fusion was positive for 3 cases (11.1%) of p190 fusion and 2 cases (7.4%) of p210 fusion in B + My cases. WHO 2016 criteria ar...Continue Reading
References
Dec 22, 1999·Leukemia·J J van DongenA Biondi
Jan 10, 2009·Blood·Jeffrey E RubnitzChing-Hon Pui
Apr 10, 2009·Blood·James W VardimanClara D Bloomfield
Aug 29, 2009·Haematologica·Amal S Al-SeraihyAsim F Belgaumi
Sep 17, 2010·Leukemia·O K Weinberg, D A Arber
Jan 14, 2011·Blood·Estella MatutesWolf-Dieter Ludwig
Jan 27, 2011·Acta Haematologica·Yanming ZhangYue Han
Oct 22, 2011·International Journal of Hematology·Ying WangJianxiang Wang
May 15, 2012·Haematologica·Lingzhi YanSuning Chen
Jan 18, 2013·Cytometry. Part B, Clinical Cytometry·Willemijn van den AnckerArjan A van de Loosdrecht
Oct 23, 2013·Annals of Hematology·Marcela Deffis-CourtErick Crespo-Solís
Feb 18, 2014·International Journal of Hematology·Chika KawajiriChiaki Nakaseko
Jan 22, 2015·Blood·Ofir Wolach, Richard M Stone
Aug 16, 2015·American Journal of Clinical Pathology·Anna Porwit, Marie C Béné
Dec 30, 2015·Leukemia·H GisslingerJ Thiele
Apr 14, 2016·Blood·Daniel A ArberJames W Vardiman
Jan 17, 2017·Blood Research·Hee-Je Kim
Mar 25, 2017·American Journal of Clinical Pathology·Matthew J OberleyMaurice R G O'Gorman
Jul 12, 2017·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Bartlomiej M GettaBrian C Shaffer
Oct 27, 2017·Archives of Pathology & Laboratory Medicine·Nathan J Charles, Daniel F Boyer
Dec 11, 2017·Blood·Sunil S RaikarWilliam G Woods
Mar 2, 2018·Oncotarget·Andrés E QuesadaChi Young Ok
Citations
Apr 30, 2019·Cytometry. Part B, Clinical Cytometry·Anna Porwit, Marie C Béné
Mar 31, 2020·European Journal of Haematology·Olga Zając-SpychałaJacek Wachowiak
Jun 28, 2021·International Journal of Hematology·Kangkang LvYuejun Liu