Mixed vector immunization with recombinant adenovirus and MVA can improve vaccine efficacy while decreasing antivector immunity.

Molecular Therapy : the Journal of the American Society of Gene Therapy
Arturo Reyes-SandovalAdrian V S Hill

Abstract

Substantial protection can be provided against the pre-erythrocytic stages of malaria by vaccination first with an adenoviral and then with an modified vaccinia virus Ankara (MVA) poxviral vector encoding the same ME.TRAP transgene. We investigated whether the two vaccine components adenovirus (Ad) and MVA could be coinjected as a mixture to enhance protection against malaria. A single-shot mixture at specific ratios of Ad and MVA (Ad+MVA) enhanced CD8(+) T cell-dependant protection of mice against challenge with Plasmodium berghei. Moreover, the degree of protection could be enhanced after homologous boosting with the same Ad+MVA mixture to levels comparable with classic heterologous Ad prime-MVA boost regimes. The mixture increased transgene-specific responses while decreasing the CD8(+) T cell antivector immunity compared to each vector used alone, particularly against the MVA backbone. Mixed vector immunization led to increased early circulating interferon-γ (IFN-γ) response levels and altered transcriptional microarray profiles. Furthermore, we found that sequential immunizations with the Ad+MVA mixture led to consistent boosting of the transgene-specific CD8(+) response for up to three mixture immunizations, whereas each ...Continue Reading

References

Nov 14, 1997·Nature Biotechnology·S C GilbertA V Hill
Nov 24, 1999·Immunological Reviews·J SchneiderA V Hill
Nov 12, 2003·Cellular Immunology·A R PintoH C J Ertl
Jul 23, 2004·The Journal of General Virology·Robert DrillienDaniel Hanau
Dec 9, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hoyin MokMichael A Barry
Mar 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Daniel P WebsterAdrian V S Hill
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carla OseroffAlessandro Sette
Oct 26, 2005·Molecular and Biochemical Parasitology·Chris J JanseAndrew P Waters
Jun 16, 2006·Journal of Virology·David C TscharkeJonathan W Yewdell
Apr 28, 2007·Immunology·Arturo Reyes-SandovalStephen M Todryk
Feb 13, 2008·European Journal of Immunology·Arturo Reyes-SandovalAdrian V S Hill
Jul 10, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Reinhold SchirmbeckFlorian Kreppel
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jun 11, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Marcio O Lasaro, Hildegund C J Ertl
Oct 9, 2009·Journal of Visualized Experiments : JoVE·Peter D BurbeloMichael J Iadarola
Oct 22, 2009·The New England Journal of Medicine·Supachai Rerks-NgarmUNKNOWN MOPH-TAVEG Investigators
Nov 20, 2009·PloS One·Ivo H J PloemenBlandine M D Franke-Fayard
Jan 12, 2010·Human Vaccines·Adrian V S HillSimon J Draper
Jun 4, 2010·Expert Review of Vaccines·Peter D BurbeloMichael J Iadarola
Jan 26, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mario RoedererMartha C Nason
Apr 26, 2011·Current Opinion in Immunology·Christine S RollierAdrian V S Hill
Jul 1, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arturo Reyes-SandovalAdrian V S Hill

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Citations

Jan 23, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R F O FrançaS O De Paula
May 9, 2013·Expert Opinion on Biological Therapy·Leo SwadlingEleanor Barnes
Sep 2, 2015·Vaccine·Stephen L HoffmanPatrick E Duffy
Nov 27, 2014·Human Vaccines & Immunotherapeutics·Juan García-Arriaza, Mariano Esteban
Sep 9, 2016·Expert Review of Vaccines·Michael R HollingdaleKeith Limbach
Feb 10, 2017·Clinical and Vaccine Immunology : CVI·Eduardo AlvesArturo Reyes-Sandoval
Aug 5, 2016·Clinical and Experimental Vaccine Research·Hong-Tae Park, Han Sang Yoo
Aug 6, 2017·Scientific Reports·Anita MilicicArturo Reyes-Sandoval
Oct 31, 2013·Expert Review of Vaccines·Carmen Elena GómezMariano Esteban
Nov 22, 2016·Clinical & Translational Immunology·Lauren E HolzWilliam R Heath

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Datasets Mentioned

BETA
GSE30564

Methods Mentioned

BETA
transgenic
biopsies
biopsy
chemical
Assay
immunoprecipitation
flow cytometry
chips
chip

Software Mentioned

Graphpad
FlowJo Tree Star
Living Image
Illumina
limma
Beadstudio
Bioconductor
FlowJo
Pestle
SPICE

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