Mks1p is required for negative regulation of retrograde gene expression in Saccharomyces cerevisiae but does not affect nitrogen catabolite repression-sensitive gene expression.

The Journal of Biological Chemistry
Jennifer J TateTerrance G Cooper

Abstract

The Tor1/2p signal transduction pathway regulates nitrogen catabolite repression (NCR)-sensitive (GAP1, GAT1, DAL5) and retrograde (CIT2, DLD3, IDH1/2) gene expression by controlling intracellular localization of the transcription activators, Gln3p and Gat1p, and Rtg1p and Rtg3p, respectively. The accepted pathway for this regulation is NH(3) or excess nitrogen dash, vertical Mks1p dash, vertical Ure2p dash, vertical Gln3p --> DAL5, and rapamycin or limiting nitrogen dash, vertical Torp --> Tap42 dash, vertical Mks1p --> Rtg1/3p --> CIT2, respectively. In current models, Mks1p positively regulates both Gln3p (and DAL5 expression) and Rtg1/3p (and CIT2 expression). Here, in contrast, we show the following. (i) Mks1p is a strong negative regulator of CIT2 expression and does not effect NCR-sensitive expression of DAL5 or GAP1. (ii) Retrograde carbon and NCR-sensitive nitrogen metabolism are not linked by the quality of the nitrogen source, i.e. its ability to elicit NCR, but by the product of its catabolism, i.e. glutamate or ammonia. (iii) In some instances, we can dissociate rapamycin-induced CIT2 expression from Mks1p function, i.e. rapamycin does not suppress Mks1p-mediated down-regulation of CIT2 expression. These findings s...Continue Reading

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Citations

Jul 26, 2006·The Journal of Biological Chemistry·Jennifer J TateTerrance G Cooper
Jan 22, 2005·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Irit Gat-ViksRon Shamir
Sep 25, 2010·Omics : a Journal of Integrative Biology·Luciano GaldieriAles Vancura
Mar 13, 2003·Molecular Biology of the Cell·Karen P WedamanTed Powers
Aug 12, 2005·Molecular Biology of the Cell·Zhengchang LiuRonald A Butow
Nov 1, 2006·Molecular and Cellular Biology·Debrah M Thompson, Roy Parker
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Joseph V GrayMargaret Werner-Washburne
Jun 15, 2006·Annual Review of Genetics·Zhengchang Liu, Ronald A Butow
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