Abstract
Necroptosis has been reported to be involved in cisplatin-induced cell death, but the mechanisms underlying the occurrence of necroptosis are not fully elucidated. In this study, we show that apart from apoptosis, cisplatin induces necroptosis in A549 cells. The alleviation of cell death by two necroptosis inhibitors-necrostatin-1 (Nec-1) and necrosulfonamide (NSA), and the phosphorylation of mixed lineage kinase domain-like protein (MLKL) at serine 358, suggest the involvement of receptor-interacting protein kinase 1 (RIPK1)-RIPK3-MLKL signaling in cisplatin-treated A549 cells. Additionally, the initiation of cisplatin-induced necroptosis relies on autocrine tumor necrosis factor alpha (TNF-α). Furthermore, we present the first evidence that phosphatidylinositol transfer protein alpha (PITPα) is involved in MLKL-mediated necroptosis by interacting with the N terminal MLKL on its sixth helix and the preceding loop, which facilitates MLKL oligomerization and plasma membrane translocation in necroptosis. Silencing of PITPα expression interferes with MLKL function and reduces cell death. Our data elucidate that cisplatin-treated lung cancer cells undergo a new type of programmed cell death called necroptosis and shed new light on ...Continue Reading
Citations
Oct 26, 2018·Journal of Virology·Amit GabaYan Zhou
Jun 10, 2019·Apoptosis : an International Journal on Programmed Cell Death·Lin JingZhi-Nan Chen
Aug 17, 2019·American Journal of Physiology. Heart and Circulatory Physiology·Paras K MishraTakashi Matsui
Jun 21, 2019·Marine Drugs·Shuai HaoChengtao Wang
Mar 29, 2020·Biomolecules·Brittany DewdneyLionel Hebbard
May 8, 2020·International Endodontic Journal·X DaiW Zhao
Aug 15, 2019·Experimental and Therapeutic Medicine·Aijun RenLiangjie Zheng
Aug 6, 2020·Cells·Jenny SprootenAbhishek D Garg
Dec 16, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Heng-Long WangJer-Yiing Houng
Mar 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shaoming JinFazheng Ren
Nov 14, 2019·Cell Death & Disease·Tamás MolnárGábor Koncz
Oct 21, 2018·Cell Death and Differentiation·Michelle N MessmerAndrew Oberst
Aug 30, 2019·Oncogene·Evgeniia A ProkhorovaGelina S Kopeina
Jul 15, 2020·AAPS PharmSciTech·Laxmikant GautamS P Vyas
Mar 8, 2020·Cell Death and Differentiation·Eva BrackBeat W Schäfer
Aug 13, 2020·Cancers·Caroline ChangNadine Hempel
Nov 17, 2020·Environmental Pollution·Lu PengYang Song
Nov 10, 2020·Pharmacology & Therapeutics·Imran KhanIIana Chefetz
Apr 10, 2021·Acta Pharmacologica Sinica·Fang WangJuan Jin
Oct 17, 2020·Journal of Molecular Cell Biology·Lu LiXiawei Wei
May 7, 2021·Cell Death and Differentiation·Sofie MartensNozomi Takahashi
May 27, 2021·Acta Pharmacologica Sinica·Hong YuGuang-Biao Zhou
Jun 14, 2021·Experimental Cell Research·Jingliao Zhang, Kuanhai Wei