MMI-0100 Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice through Targeting MK2 Pathway

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Zhe WangMin Chang

Abstract

Backgrounds: This study aimed to investigate the protective effects of MMI-0100, a cell-penetrating peptide inhibitor of MAPK-activated protein kinase II (MK2), on acute colitis induced by dextran sodium sulfate (DSS). Mice were injected intraperitoneally with different doses of MMI-0100 (0.5 and 1 mg/kg per day, six days). The physiological indexes, the parameters for colonic pathological injury and the intensity of inflammatory responses were evaluated by histological staining, quantitative PCR, western blotting, and immunostaining. MMI-0100 attenuated DSS-induced body weight loss, colon length shortening, and colonic pathological injury, including decreased myeloperoxidase (MPO) and inhibited inflammatory cell infiltration. MMI-0100 suppressed DSS-induced activation of CD11b+ and F4/80 positive cell, and dramatically decreased the expression of a series of pro-inflammatory cytokines such as TNF-α, IL-6, IL-1β, TGF- β, IFN-γ, IL-17A, COX-2 and iNOS. A TUNEL assay showed that MMI-0100 protected against DSS-induced apoptosis. This is consistent with the results of Western blotting assay in apoptosis-related proteins including Bcl-2, BAX, caspase-3. The anti-inflammatory effects of MMI-0100 on DSS-induced colitis were achieved b...Continue Reading

References

Jun 8, 2002·Molecular and Cellular Biology·Alexey KotlyarovMatthias Gaestel
Jan 21, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anne Barbara TietzClaus Schäfer
Apr 16, 2011·The Journal of Surgical Research·Brian C WardAlyssa Panitch
May 24, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jamie L BrugnanoAlyssa Panitch
Oct 1, 2011·Journal of Digestive Diseases·Ya Jing Feng, Yong Yu Li
May 17, 2014·World Journal of Gastroenterology : WJG·Éva Pallagi-KunstárTamás Molnár
Sep 2, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Puneet Kaur RandhawaAmteshwar Singh Jaggi
Jun 17, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Klara SoukupAlexander M Dohnal
Apr 15, 2016·Cell Host & Microbe·Alexander R MoschenHerbert Tilg
May 26, 2016·Nucleic Acids Research·Christopher TiedjeMatthias Gaestel
Dec 16, 2016·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Wen-Feng ZhangMing-Hua Duan
May 17, 2017·Molecular Cell·Isabel JacoPascal Meier
Jul 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ling ZhaoZhao-Xiang Bian
Sep 6, 2017·Journal of the American Heart Association·Qinghang MengJeffrey Robbins
Oct 11, 2017·International Journal of Peptide Research and Therapeutics·David I BrownMonte S Willis
Dec 26, 2017·Trends in Biochemical Sciences·Manoj B Menon, Matthias Gaestel
Feb 27, 2018·Pharmacological Reports : PR·JinHong JiangMin Chang
Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan-Fang JiaoJian-Qiang Yu
Apr 4, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ae Sin LeeYu Jin Jung
Jul 15, 2018·British Journal of Pharmacology·Carlos J ArandaFermín Sánchez de Medina
Aug 22, 2018·American Journal of Respiratory Cell and Molecular Biology·Jiurong LiangPaul W Noble
Sep 12, 2018·Pulmonary Pharmacology & Therapeutics·Mariam BaCherie A Singer
Sep 14, 2018·European Journal of Gastroenterology & Hepatology·Julian EssmannOliver Bachmann
Sep 30, 2018·Current Gastroenterology Reports·Christopher M Johnson, Themistocles Dassopoulos

❮ Previous
Next ❯

Citations

Feb 20, 2021·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Jing ChengLi Liu

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
protein assay

Clinical Trials Mentioned

NCT01937338
NCT03435861
NCT00559910
NCT02515396

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis