MmNEU3 sialidase over-expression in C2C12 myoblasts delays differentiation and induces hypertrophic myotube formation

Journal of Cellular Biochemistry
Nadia PapiniBruno Venerando

Abstract

Several factors affect the skeletal muscle differentiation process, in particular modifications of cell-cell contact, cell adhesion, and plasma membrane characteristics. In order to support the role of the plasma membrane-associated sialidase NEU3 in skeletal muscle differentiation and to analyse which events of the process are mainly affected by this sialidase, we decided to stably over-express MmNEU3 in C2C12 cells by a lentiviral vector and to investigate cell behavior during the differentiation process. Vitally stained C2C12 and NEU3 over-expressing cells were counted to reveal modifications in differentiation induction. We found that NEU3 over-expressing cells remained proliferative longer than control cells and delayed the onset of differentiation. Expression of p21, myogenic transcription factors, and myosin heavy chain (MHC), assessed by real time PCR, confirmed this behavior. In particular, no MHC-positive myotubes were present in NEU3 over-expressing cells as compared to wild type C2C12 cells at day 3 of differentiation. Moreover, NEU3 over-expressing cells completed the differentiation process very quickly and formed hypertrophic myotubes. Analysis of MAPK/ERK pathway activation showed an increased ERK 1/2 phosphoryl...Continue Reading

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Citations

Nov 2, 2012·Skeletal Muscle·Alessandro FanzaniEugenio Monti
Jan 16, 2019·Cell Death and Differentiation·Jia LiuGuiling Wang
Mar 19, 2016·The Journal of Biological Chemistry·Federica CirilloLuigi Anastasia
Sep 14, 2018·Stem Cells International·Sonia BerganteLuigi Anastasia
Nov 30, 2016·Journal of Cachexia, Sarcopenia and Muscle·Christopher Lipina, Harinder S Hundal
Sep 2, 2020·The Biochemical Journal·Andrea GhiroldiLuigi Anastasia
Dec 28, 2017·Cancer Biomarkers : Section a of Disease Markers·Matilde ForcellaMilo Frattini
Jun 25, 2017·Scientific Reports·Macarena Rodriguez-Walker, Jose L Daniotti
Oct 9, 2020·Journal of Muscle Research and Cell Motility·Kapila AwasthiAlok Bhattacharya
Jan 17, 2020·International Journal of Biological Macromolecules·Sabina LipničanováStanislav Miertuš
Jul 7, 2018·Chemical Reviews·Carmanah D HunterChristopher W Cairo

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