MMP-11 promotes papillary thyroid cell proliferation and invasion via the NF-κB pathway

Journal of Cellular Biochemistry
Zhun ZhangShili Liu

Abstract

Papillary thyroid carcinoma (PTC) is the most common form of thyroid cancer, and its incidence is on the rise. It has been reported that some matrix metalloproteinases (MMPs) are abnormally expressed in PTC and can be used as diagnostic markers. However, few studies have explored the underlying mechanisms by which MMPs promote tumor progression. In this study, we used microarray analysis to compare the variations of gene expression within the PTC cell populations and their adjacent normal tissues and found that MMP-11 was the most differentially expressed MMP. To investigate the role of MMP-11 in the mediation of thyroid cancer cell development, pEnter-MMP-11 plasmid, and MMP-11 small interfering RNA were applied to up- and downregulate MMP-11 expression of in cultured PTC cell lines K1 and BCPAP. The results suggested that the levels of proliferation and migration of cells transfected with MMP-11 siRNA were significantly reduced, while the levels in MMP-11-plasmid-transfected cells were increased. In terms of the mechanism, experimental data showed that the change in cyclin D1 is consistent with MMP-11 expression, which may explain the changes in proliferation. In addition, Western blot assay was conducted to analyze the p65 a...Continue Reading

References

Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C WolfP Basset
Mar 1, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·P BassetM C Rio
Jul 20, 1999·Molecular and Cellular Biology·D C GuttridgeA S Baldwin
Feb 24, 2001·Virchows Archiv : an International Journal of Pathology·J MuellerH Höfler
Feb 22, 2002·Cancer·Anne Gentil PerretJean-François Mosnier
Apr 30, 2002·International Journal of Cancer. Journal International Du Cancer·Pia Vihinen, Veli-Matti Kähäri
Sep 2, 2003·International Journal of Cancer. Journal International Du Cancer·Shilpi SoniRanju Ralhan
Nov 19, 2004·Nature·Neil A BhowmickHarold L Moses
Mar 25, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shilpi AroraRanju Ralhan
Oct 19, 2006·Science's STKE : Signal Transduction Knowledge Environment·Hans Häcker, Michael Karin
Jan 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yan-Hong YangYou-Yong Lu
Nov 4, 2008·Biological Chemistry·Elena Roza Motrescu, Marie-Christine Rio
Apr 21, 2009·Biochimica Et Biophysica Acta·Cynthia TallantF Xavier Gomis-Rüth
May 30, 2009·International Journal of Cancer. Journal International Du Cancer·Daniel BuergyHeike Allgayer
Nov 4, 2011·The Journal of Clinical Endocrinology and Metabolism·Maqbool AhmedKhawla S Al-Kuraya
Oct 20, 2012·Cancer Cell·Yoon Jong ChoiPiotr Sicinski
May 17, 2013·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Briseis Aschebrook-KilfoyRaymon H Grogan
Feb 22, 2014·JAMA Otolaryngology-- Head & Neck Surgery·Louise Davies, H Gilbert Welch
Oct 15, 2014·Immunology Letters·Nina Milutin GašperovMagdalena Grce
Jun 23, 2015·Genes & Diseases·Jillian CathcartJian Cao
Oct 4, 2016·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Shuo Qie, J Alan Diehl
Jul 22, 2017·International Journal of Oncology·Lei-Lei HuangLian-Tang Wang

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