MMP-9 mRNA as a therapeutic marker in acute and chronic stages of arthritis induced by type II collagen antibody

Journal of the Formosan Medical Association = Taiwan Yi Zhi
Wei-Tso ChiaHuey-Kang Sytwu

Abstract

Antibodies against type II collagen (anti-CII) are arthritogenic and central to the initiation of the disease. An animal model of collagen type II-specific monoclonal antibody-induced arthritis (CAIA) has been used for the evaluation of various therapeutic effects in rheumatoid arthritis (RA). We aimed to measure the expression of matrix metalloproteinase (MMP)-9 (gelatinase B), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in the acute and chronic stages of the CAIA model for application as a therapeutic marker. A commercially available antibody cocktail containing four monoclonal anti-type II collagen antibodies were injected into 6 to 8-week-old male BALB/c mice (n=20) and 50 microL lipopolysaccharide was injected 3 days later. The clinical manifestations of RA were recorded and scored at 10 days (acute stage) and 21 days (chronic stage). Then the mice were sacrificed for histologic analysis of the inflamed footpad and gene expression of IL-1beta, TNF-alpha and MMP-9 by ELISA and quantitative polymerase chain reaction amplification. Marked inflammation was found in the limb joints of mice at 10 days. Both IL-1beta and MMP-9 expression played a central role in the inflammatory reaction in the acute ...Continue Reading

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Citations

Dec 1, 2009·Medicina clínica·Sol de la PeñaKenneth León-Córdoba
Apr 16, 2013·Scandinavian Journal of Rheumatology·C YeN Manjunath
Dec 2, 2010·The Journal of Bone and Joint Surgery. British Volume·W-T ChiaH-K Sytwu
Jun 3, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Abdulaziz M AldayelZhengrong Cui

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