MMP2-sensing up-conversion nanoparticle for fluorescence biosensing in head and neck cancer cells

Biosensors & Bioelectronics
Yung-Chieh ChanMichael Hsiao

Abstract

Upconversion nanoparticles (UCNPs) have extensive biological-applications because of their bio-compatibility, tunable optical properties and their ability to be excited by infrared radiation. Matrix metalloproteinases (MMPs) play important roles in extracellular matrix remodelling; they are usually found to significantly increase during cancer progression, and these increases may lead to poor patient survival. In this study, we produced a biosensor that can be recognized by MMP2 and then be unravelled by the attached quencher to emit visible light. We used 3.5-nm gold nanoparticles as a quencher that absorbed emission from UCNPs at a wavelength of 540 nm. The biosensor consists of an upconversion nanoparticle, MMP2-recognized polypeptides and quenchers. Here, UCNPs consisting of NaYF4:Yb(3+)/Er(3+) were prepared via a high temperature co-precipitation method while protecting the oleic acid ligand. To improve the biocompatibility and modify the UCNPs with a polypeptide, they were coated with a silica shell and further conjugated with MMP-recognizing polypeptides. The polypeptide has two ends of featuring carboxylic and thiol groups that react with UCNPs and AuNPs, and the resulting nanoparticles were referred to as UCNP@p-Au. Ac...Continue Reading

References

Jul 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N ThéretB Clément
Nov 6, 2001·The Journal of Biological Chemistry·Emily I ChenJeffrey W Smith
Feb 10, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jennifer A KwanRichard Schulz
Dec 2, 2011·Chemical Society Reviews·Lev Dykman, Nikolai Khlebtsov
Jun 28, 2012·Sensors·Jiao Chen, Julia Xiaojun Zhao
Nov 1, 2012·Biotechnology Advances·Anil KumarXing-Jie Liang
Nov 8, 2014·Molecular Cancer Therapeutics·Lindsey N MicelS Gail Eckhardt
Jan 24, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yang ShiJianhua Qin
Mar 7, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Alireza LotfiLale Saniee
Apr 8, 2015·Cancer Chemotherapy and Pharmacology·Rongbao ZhaoI David Goldman
Jun 20, 2015·International Journal of Cancer. Journal International Du Cancer·Jheelam BanerjeeHildegard M Schuller
Jun 27, 2015·ACS Applied Materials & Interfaces·Rui WangHaimei Gong
Aug 19, 2015·Chemical Communications : Chem Comm·Shi-Ying LiXian-Zheng Zhang
Sep 1, 2015·Archives of Medical Research·Zahra AdabiMandana Hasanzad
Sep 28, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Han XuQingxia Fan
Nov 12, 2015·Contemporary Oncology Współczesna Onkologia·Haniehsadat EftekharyMojgan Nassiri

❮ Previous
Next ❯

Citations

Aug 18, 2017·Nanomedicine·Yung-Chieh Chan, Michael Hsiao
Nov 22, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhen LeiZhenxin Wang
Feb 28, 2021·Molecular Biotechnology·Jaya SinghalDivya Mehrotra
May 14, 2021·Bioinorganic Chemistry and Applications·Weiping ZhengChangqing Yuan
Dec 10, 2016·ACS Applied Materials & Interfaces·Chieh-Wei ChenRu-Shi Liu
Aug 29, 2021·Pharmaceuticals·Gustavo MoraesWalter L Siqueira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.

Cancer Cell Invasion: Nanomedicine

Nanomedicine is a promising alternative for cancer detection and therapy that utilizes nanoparticles, such as liposomes. Nanoparticles can potentially target cancer cell invasion and metastasis. Discover the latest research on Cancer Cell Invasion: Nanomedicine here.